| Background:Epilepsies comprise the second most common group of neurologic disorders after stroke.Therefore,how to prevent and treat epilepsy with its high morbidity and mortality becomes important. The existing antiepileptic drugs can not prevent and cure epilepsy completely for some reasons such as their simplex mechanism.In order to prevent and cure epilepsy entirely,we should take various measures to intervene in epilepsy.Ginkgo biloba extract (GBE) is composed of multipicate components,so it may affect various targets and prevent and treat epilepsy more effectively. Objective:In order to evaluate the effects of GBE on epilepsy and discuss the possible mechanism,we employed Lithium-Pilocarpine Seizures(LPS) rat model to investigate the effects of GBE on behaviors,electroencephalogram(EEG) and the neurons and the immunoreactive of microglia in cerebellum of LPS rats throuh pre-treatment and post-treatment administration modes.We expect to put forward a more safety and effective drug for the prevention and treatment of epilepsy and provide experimental proof and guidance for the application of GBE preparation to the prvention and trdatment of clinical epilepsy. Method:We selected healthy male adult Sprague-Dawley white rats as research objects.All drugs were administered intraperitoneally.One hundred and twenty rats were divided into five groups randomly:normal saline control group(n=6),GBE contorl group(n=6),LPS group(n=36),GBE pre-treatment group(n=36),GBE post-treatment group(n=36).The latter three group were divided into six subgroups according to the different sacrificed time point:that is 3 hours(3h), 1 day(1d),3 days(3d),7 days(7d),14 days(14d),60 days(60d) after pilocarpine adimistration,each subgroup is composed of six rat.The changes of behaviors and EEG of rats from varios groups were recorded and the sections in cerebellum were Nisse-stained and immunohistochemistry-strained by c-Fos prtein and Griffonia Simplicifoia isolectin B4(GSA-B4). Results: 1.Bevaiors: normal saline contronl group and GBE control group:all 0 stage.The rate of seizure stage≥4 of LPS group,GBE pre-treatment group and GBE post-treatment group was 91.67%,5.56% and 86.11% respectivel.The mortality of LPS group,GBE pre-treatment group and GBE post-treatment group was 11.11%,0 and 8.33% respectively.The rearing latency of LPS group, GBE pre-treatment group and GBE post-treatment group was 30.38±12.01 min,95.00±46.67 min and 30.75±12.92 min respectively.There were significant differences in the rate of seizure stages≥4 and seizure stages between GBE pre-treatment group and LPS group.The effective rate of GBE pre-treatment and post-treatment adiminstration against LPS was 94.44% and 35.48% respectively. 2.EEG: The EEG of normal saline control group and GBE control group was normal.The frequency was increased and the amplitute of waves was elevated on the EEG of LPS group.And there were multiple spikes waves discharges or paroxysmal and continual spikes clusters discharges on the EEG of LPS group.There was only increasing frequency and but no spike dischages on the EEG of pre-treatment group.The EEG of post-treatment group is similar to that of LPS groups. 3.Pathomorphology (1)Nissl staining: Compared with normal saline control group,there were no neuron loss in cerebellum of LPS group,GBE pre-treatment group and GBE post-treatment group rats. (2)c-Fos protein immunohistochemistry staining:There were no c-Fos protein positive cells in the cerebellum of all the five groups rats. (3)GSA-B4 immunohistochemistry staining:There were no GSA-B4 positive cells in the cerebellum of normal saline control group and GBE control group rats.Compared with LPS group,the number of GSA-B4 positive cells in cerebellum of GBE pre-treatment group and GBE post-treatment group decreased significantly and the optical mean density of GSA-B4 postitive cells increased significantly. Conclusion: 1.There are different phases that appears sequently in LPS rat model.And it can be operated simply,observed clearly and easily ,cheap.So it is an idea...
|
PDF Full Text Request