Chemical Constituents From Incarvillea Arguta Royle

Incarvillea arguta Royle., belonging to the Bignoniaceae family, grows in an altitude of 1400-2700 meters area in Lancang and Jinsha River basin, and mainly distributes in Sichuan, Guizhou, Gansu, Tibet and Yunnan provinces. It is also called as “Li-La-Wei”, “Wa-Bu-You” and “Chao-Tian-Gui” in Yi Medicine. I. arguta possessed many bioactivity, the dry or fresh whole plant could be used for the treatment of cholecystitis, gall-stone, and kidney stone. The extract of the whole plant could also be employed for the treatment of hepatitis and bacillary dysentery. Many chemical constituents involving iridoids, sesquiterpenois, triterpenoids, steroids,alkaloids, flavonoids, amides, and phenylpropanoids had been reported from this medicinal plant.To find a bioactivity constituent from this medicinal plant, a phytochemical investigation on I. arguta was conducted and 21 compounds were isolated from the title plant. By comprehensive analysis of the1H-NMR,13C-NMR,1H-1H COSY,HSQC, HMBC, ROESY, MS spectra, the 21 isolates were determined as 5-hydroxy-7,4’-dimethoxy- 6-methyl-flavone(1), 5-hydroxy- 7,4′-dimethoxy- 6,8-methyl-flavone(2), 3β-hydroxy- 11-ursen- 28,13-olide(3), fupenzic acid(4),argutosine B(5), incarvilleaol(6), argutine C(7), delavayine D(8), delavayine E(9),boschniakinic acid(10),(3R,7S)- 3,7-dimethyl- 8-hydroxyoctanoic octyl acetate(11),(2S,6R)- 2,6-dimethyl- 1,8-octanediol(12), ursolic acid,(13), oleanolic acid(14), β-sitosterol(15); incargutosine C(16); argutane C(17); argutane B(18);daucosterol(19), argutalactone(20), 2- methoxy- 5- hydroxy benzaldehyde(21)。The isolates included iridoids, sesquiterpenois, triterpenoids, steroids, alkaloids, and flavonoids. Compound 7 was a new one and compounds 4、8、9、11、12、21 were isolated from this plant for the first time.