Study On Intestinal Microecology Recovery And Re-establishment By Using Apple Enzyme In Mice Model

Objective:Intestinal dysbacteriosis model was established in mice. Study on intestinal microecology recovery and re-establishment used by Apple enzyme in mice model from the perspective of microecology. To find a safe and efficient probiotics treatment of intestinal dysbacteriosis and to provide a theory basis.Methods:1. 50 Kunming mice that 8 weeks, average weight 33.28±3.13 g, male and female each half were fed in cage in a clean environment. Randomly selected 10 mice as common control group, the 40 mice to establish animal model.2. Using lincomycin hydrochloride lead to antibiotic associated diarrhea in mice. Selected randomly 10 mice in testing. After being dysbacteriosis model, random 30 mice can be divided into model group, treatment group, probiotics group, 10 mice in each group.3. Using apple enzyme to intervene, mice feces in the ileum and colon were collected sterilely. The total DNA was extracted and tested by PCR-DGGE, through comparing grey value in the band.3. Cluster analysis, similarity analysis, diversity analyzing and statistical method were used.Results:1. After be received lincomycin hydrochloride, all the mice showed diarrhea symptom that feces thin, shapeless and yellow water samples aggravate gradually, poor state of mental and behavior, and appear different degree of depression at the same time, eat less, slow activity, chills cluster. The average weight by building the highest weight 35.69 ± 1.32 g dropped to 30.52 ±0.72 g, compared with common control group of mice, there was statistically significant difference(R < 0.01). The DGGE profiles of model group mice of ileum and colon dominant microbiota displayed the quantity and brightness of the stripe in the lane less than the common control group significantly, intestinal microbiota diversity index decreased, compared to the corresponding control group, there was statistically significant difference(R < 0.05).2. Each group was accepted different intervention After 7d, probiotics group and experimental group mice behave gradually active, have a good mental condition and relieve diarrhea symptoms. The characters of feces change into yellow, granular, hard. The food intake increase gradually, and faster than model group recovery. Experimental group mice weight is the fastest in all groups, at 35.35±2.56 g, compared with weight before treatment(R < 0.01) and model group(R < 0.05), there was statistically significant difference. Compared with common control group(R > 0.05) and probiotics group(R > 0.05), there was no statistically significant difference.3. The DGGE profiles of ileum and colon dominant microbiota of common control group mice shows that there is a large number of bands in lane. For the same samples, no obvious difference was found between the position and brightness of the strip in the lane. Between different parts of the sample, location and brightness in the band have an obvious difference. In common control group, Colonic dominant microbiota diversity is higher than the ileum, there was statistically significant difference(R < 0.05).4. The DGGE profiles of ileum and colon dominant microbiota of each group mice with Apple enzymes showed that probiotics group and experimental group with the number average more than model group. There are some common bands in each group, at the same time, some differences was observed in all the strips. The similarity analysis and cluster analysis show the lanes of the experimental group and common control group the highest similarity, and together as one family. The diversity and richness of each groups were lower than the common control group, there was statistically significant difference(R < 0.05). The richness and diversity of probiotics group and experimental group were greater than model group, there was statistically significant difference(R < 0.05). Experimental group slightly better than the probiotics group, but there was no statistically significant difference.Conclusion:Intestinal dysbacteriosis was established in mice, PCR-DGGE was adopted to detect intestinal microecology in mice model. It is find that apple enzymes has the effect of and re-establishment on intestinal dysbacteriosis.