Relative Research Between Oxytocin Receptor And Adenomyosis

Object:To detect expression changes of Oxytocin Receptor(OTR) in endometrial-myometrial interface(EMI)、smooth muscle tissue of thickened myometrium and cervical tissue in patients with adenomyosis(AM), then explore the pathogenesis of AM and dysmenorrheal and intensity changes of uterine contractions in patients with dysmenorrheal.and make early exploration about the issue of cervical reservations in Surgical treatment.Method:Sixty-nine AM cases(20 with serious dysmenorrheal,19with moderate dysmenorrheal,17 with mild dysmenorrheal,13with no pain)-.20 uterine myoma cases and 20 cervical III cases were collected from September 2013 and March 2015 who admitted to department of gynecology in our hospital and received hysterectomy. Select tissues of EMI、thickened myometrium and cervical tissue in 69 AM patients. Similarly, in control groups, tissues of EMI and thickened myometrium were selected from 20 cervical III cases and cervical tissue were selected from 20 uterine myoma cases. All the patients were pathologically confirmed diagnosis. All specimens were fixed in 10% formalin, routinely dehydrated, embedded in paraffin, and cut into small sections, Immunohistochemical method was used to identify the expression of oxytocin receptor in different tissues. The expression level was detected by using digital image analysis with optical microscope and Image Pro-Plus 6.0.Results:(1)OTR protein expression of EMI in AM group was significantly higher than in the control group, and the differences were statistically significant. The shape of EMI in AM group is irregular, and part of the basal endometrial glands were interspersed into the superficial muscle cracks continuously. Besides, OTR protein expression in irregular zone of EMI was higher than the adjacent regular zone, and the differences were statistically significant.(2)AM group were divided into different groups by degrees of dysmenorrheal. By Detecting OTR protein expression of EMI in these groups, we found that OTR protein expression in each AM group were significantly higher than the controls. However, with increasing intensity of dysmenorrheal, OTR protein levels did not show a continuous upward trend, and no significant difference among the AM groups.(3)The positive expression rates of OTR in smooth muscle of myometrium were higher than that in controls, and the expression level increased with the aggravation of dysmenorrheal. All the differences were statistically significant.(4)No ectopic endometrial tissue were seen in cervical tissues, and OTR protein does not expressed in the cervical mucous glandular epithelium and stroma among each degree of dysmenorrheal group and control group.(5)OTR protein were positive expressed in the vascular endothelial cells and smooth muscle cells of cervical tissues in each degree of dysmenorrheal group and control group, but there was no significant difference between groups.Conclusions:(1)The increased expression of OTR in EMI and basal endometrial retraction may be associated with the pathogenesis of adenomyosis.(2)The expression level of OTR in smooth muscle of myometrium is positively correlated with the degree of dysmenorrhea. This indicates that the increased intensity of uterine contraction is involved in the pathogenesis of dysmenorrhea.(3)No ectopic endometrial tissue were seen in cervical tissues, and no significant difference among different degree of dysmenorrheal groups and control groups in cervical tissues with the expression of OTR protein.