Expression Levels And Clinical Significance Of Type Ⅰ Innate Lymphocytes And Related Cytokines In Primary Immune Thrombocytopenia

Objective: The pathogenesis of primary immune thrombocytopenia is complex,and a variety of evidence suggests that its pathogenesis is related to T cell subset imbalance,humoral immunity,immune tolerance and autoantibody formation,resulting in excessive platelet clearance and production disorders.Type I intrinsic lymphocytes are a group of innatelike lymphocytes that do not express a variety of antigen receptors distributed by clonal distribution,but have similar morphology and secretion of cytokines similar to T and B lymphocytes,and participate in the pathogenesis of a variety of autoimmune diseases.Therefore,in order to explore the immune response of ITP pathogenesis,the role and clinical significance of ILC1 cells in ITP disease were analyzed,and the role and clinical significance of ILC1 cells in ITP disease were compared and analyzed by comparing and analyzing the transcription level and cytokines of the main transcription factor T-bet m RNA with healthy control groups and treatment groups.Methods: 30 newly diagnosed patients with ITP from June 2021 to October 2022 who met the diagnostic criteria of the 2020 edition of the ITP diagnosis and treatment expert guide were collected,and the changes of the expression level of ILC1,IL-12,IL-15,IL-18,T-bet m RNA,INF-γ were monitored by ELISA,flow cytometry,and q RT-PCR,and statistical analysis was performed.Results: The expression of ILCs and ILC1 in ITP patients was increased compared with that of healthy subjects,and their expression levels recovered after treatment,and the expression of IL-12,IL-18 and INF-γ was increased compared with that of healthy physical examiners,and the expression decreased after treatment,and there was no statistical difference.There was no statistically significant difference in IL-15 in patients with treatment-na?ve ITP compared with healthy patients.T-bet m RNA expression was increased compared with healthy subjects,and expression decreased significantly after treatment,which was statistically significant.ILC1,IL-12,INF-γ were inversely correlated with platelet count in patients with ITP before treatment.Conclusion:Patients with ITP have dysregulation of ILC1 cells,and the cytokines secreted by them are considered pro-inflammatory stimulating factors to participate in the immune response to ITP disease and are related to the degree of course of primary immune thrombocytopenia.The expression of T-bet m RNA,the main transcription factor of ILC1,was increased,which was involved in the pathogenesis of ITP.