Evaluation Of Pro-inflammatory Effect Of Litchi Extract And Citrus Citsh1

Litchi and citrus are widely accepted by consumers, because they have abundant nutrients and special functional components which are benefit for human healthy. But unfortunately, too much eating litchi and citrus fruits may cause a series of “shanghuo” symptoms, including itchy throat, sore throat, gum swelling, oral cavity ulcer, even fever and inflammation. These adverse reactions significantly impair the life quality of a large population and restrict consumption of litchi and citrus.Based on the previous researchers, we select Litchi and Satsuma as materials to evaluate the proinflammatory effects of Litchi extract and Citsh1 in murine macrophage cells and mouse.Extraction, functional assessment and identification of proinflammatory effect components from Litchi fruit:1. Extraction process of proinflammatory effect components was preliminarily established and water-soluble protein(LWP) was extracted from litchi. The specific method was as follows: Firstly, water-soluble extract(LWE) of litchi pulp was obtained after the process of juicing, filtrating, centrifugating and freeze drying. Secondly, water-soluble extract(LWE) was dialyzed and freeze-dried into powder, namely high molecular water-soluble extract(HLWE). Finally, LWP was extracted from HLWE.2. The proinflammatory effect of the LWP fractions were evaluated in RAW264.7 murine macrophage cell lines. The expressions of proinflammatory factors containing IL-1β, i NOS, COX-2 and anti-inflammatory cytokine HO-1 increased significantly in LWP-treatment group.3. The LWP fractions were identified by mass spectrometry, and then the results of mass spectrum were analyzed using SMART program and sequence alignment of homologous proteins. It showed that 14-3-3-like protein GF14 lambda, 14-3-3-like protein GF14 omega and 14-3-3-like protein GF14 upsilon were the most possible candidate proteins with proinflammatory effect than other proteins detected.Proinflammatory evaluation of citrus juice and r Citsh1.3-3 in vivo:1. Blood routine examinations of mouse were conducted after a certain period of medicining. The results showed that NE%(neutrophils ratio) and BA%(basophils percentage) increased, and LY%(lymphocytes ratio), PLT(platelet count) and PCT%(platelet deposited) decreased roughly in treatment groups.2. The mouse pathological sections of colon tissues and lung tissues showed that two kinds of tissues presented inflammatory changes in citrus juice group and r Citsh1.3-3 groups.3. The expression levels of inflammatory factors were detected in blood serum of mouse. We found that almost all five(IL-1α, IL-2, IL-4, IL-6, TNF-α) inflammatory factors had the rising tendency in citrus juice and r Citsh1.3-3 groups.