| Backgroud and Objective:Hypertensive nephropathy is one of the main complications and lethal factors of hypertension. The microalbuminuria is hypertensive nephropathy early sensitive indicator; Podocytes play a key role in the regulation of glomerular filtration in the kidney, so podocyte injury, loss, or death will leads to microalbuminuria or proteinuria. So, it suggested that the podocyte damage is one of the important causes of the proteinuria in hypertensive nephropathy. Several studies showed that 1,25(OH)2D3, an active form of vitamin D in addition to the classic regulating calcium and phosphorus metabolism to maintain normal function of the skeletal system, also has multiple immune regulating function and renoprotective function, inhibit the activity of RASS and regulating insulin secretion, regulate the function of the nervous system, etc. More and more studies shown that 1,25(OH)2D3 had renoprotective function, but the specific mechanism is not clear. We designed this experiment, in order to prove that it has protective effect on hypertensive nephropathy, and clarify the protection mechanism. It will provide new ideas for the treatment of hypertensive nephropathy.Methods:18 SHRs were randomly divided into hypertensive nephropathy group (H group), hypertensive nephropathy treated with 1,25(OH)2D3 group (D group) and normal control group (NS group), H group and NS group were given intragastric administration of equal volume of 3DW. The treated period is 16 weeks. The blood pressure (BP) of rats was measured once 4 weeks. The levels of serum albumin, serum creatinine, blood calcium, serum VitD and 24H urinary protein were measured after 16 weeks treatment. The protein level of WT1, nephrin and VDR was examined by Western blotting and immunohistochemical staining.Results:The blood pressure and serum creatinine has no obvious changes of H group and D group compared with NS group. The serum albumin, blood calcium and serum VitD levels in H group were significantly decreased compared with NS group. The level of 24H urine protein increased in H group compared with NS group, and decreased in D group compared with H group. The protein level of VDR, WT1 and nephrin were decreased in H group compared with NS group, and increased in D group compared with H group. Conclusion:1. The treatment with 1,25(OH)2D3 didn’t have effects on the blood pressure and serum creatinine in SHR. The level of 24H urine protein increased in H group. The protein expression of VDR, WT1 and nephrin were decreased in H group, whereas this reduction was attenuated by treated with 1,25(OH)2D3. We designed this experiment, in order to prove that it has protective effect on hypertensive nephropathy, and clarify the protection mechanism. It will provide new ideas for the treatment of hypertensive nephropathy. |
PDF Full Text Request