| ObjectivePre-clinical study had shown that MS sequential therapy can significantly improve the clinical symptoms of MS patients, reduce blood lipid, blood glucose and blood pressure, improve the life-quality of patients. On this basis, the project aimed to provide more reliable evidences in support of the application and new drug development by diet induction method to establish the rat model of MS, then observed the effect of MS sequential therapy of Spleen invigorating (MSI prescription)-kidney warming (MS2 prescription)-clearing (MS3 prescription)-reinforce spleen and kidney (MS4 prescription) on rat weight, blood pressure, blood lipids, and glucose, adipocytokines and primarily explore of its action mechanism.MethodsSelected 84 SD male rats(SPF), weight120-150g, after a week of adaptive feeding, According to the weight and the random number table method, divided 84 rats into normal group, model group, improve diet group and MS treatment high, medium and low dose group, positive group of Western medicine. A total of 7 groups,12 rats in each group. Normal group was given normal feed, other groups were given high fat, high sugar, high salt and high protein diet (diet induction method) continuous feeding for 8 weeks. Then each group randomly selected 2 rats detect the weight and blood pressure, fasted 12 hours, tail vein blood serum lipids and blood glucose, each group of diet induction compared with the normal group, the dif-ference was statistically significant (P<0.05) proved that the model is successful. MS treatment high, medium and low dose group, positive group of Western medicine were given intragastric administration of drug treatment, once a day, for 5 consecutive weeks, normal group, improve diet group and model group were given the same the amount of normal saline. During the experiment, model group and MS treatment high, medium and low dose group, positive group of Western medicine continue to give high fat, high sugar, high salt and high protein diet, normal control group and improve diet group were given normal feed. Testing the weight, blood pressure, blood lipids (TG, TC, HDL-C, LDL-C),and glucose, adipocy-tokines (adiponectin, leptin, TNF-α, IL-6) end of the experiment. And established a database,using SPSS17.0 statistical analysis.Results(1) The weight of MS treatment high, medium and low dose group were significantly lower than the model group, the differences were statistically significant (P<0.05). The weight of MS treatment high dose group was lower than improve diet group (P<0.05), positive group of Western medicine (P<0.01). It showed that MS sequential therapy can reduce the weightof MS rats.(2) The systolic pressure of MS treatment high, medium and low dose group, positive group of Western medicine, improve diet group were lower than the model group, the differences were statistically significant(P< 0.01). The diastolic pressure of MS treatment low dose group was lower than the model group, the difference was statistically significant (P<0.05). It showed that MS sequential therapy has good effect on blood pressure.(3) MS treatment high, medium dose group compared with the model group, the level of TC、TG、LDL-C reduced and HDL-C increased, the difference were statistically significant(P<0.05). and the level of TC of MS treatment high dose group was lower than the model group (P<0.01). It showed that MS sequential therapy has good effect on blood lipid, could reduced MS model rats’the level of TG, TC, LDL-C, and increased HDL-C content.(4) The level of fasting blood glucose of MS treatment high dose group was lower than the model group, the difference was statistically significant (P<0.05), It showed that MS sequential therapy could reduced MS model rats’ the level of fasting blood glucose.(5) MS treatment high dose group compared with the model group, the level of adiponectin increased, and leptin, TNF-α and IL-6 were decreased, the differences were statistically significant (P<0.05) It showed that MS sequential therapy could increased the level of MS model rats’ adiponectin, and reduced the level of leptin, TNF-α and IL-6.ConclusionMS sequential therapy was based on the Great Master of Chinese Medicine Guo Ziguang’s dialectical thinking in the treatment of metabolic syndrome can increase the level of MS model rats’ adiponectin, and reduce the level of leptin, IL-6 and TNF-α levels, and have the functions of regulating lipid and blood glucose, reduce obesity and blood pressure of MS model rats, which may be one of the action mechanisms of MS sequential therapy in the treatment of metabolic syndrome. This study provide a theoretical basis for further research and new drug development. |
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