Research Of The Correlation Analysis Between Kidney Injury With Other Organs’ Pathology In Different Durations Of T1dm Rat Induced By STZ

Objective: To explore the early indicators of DN lesions, and which lesion of organ keep most closely with kidney damage in T1 DM rat model. Methods: 75 male SD mice were randomly assigned into two groups, the test group(n=50), and the control group(n=25). The control groups were intraperitoneally injected with FW at a related dosage. And the SD rats in the test group were intraperitoneally injected with streptozotocin, at a dose of 60 mg/kg, to generate type 1 diabetes as DM group, which as indicated by blood glucose levels ≥16.7mmol/l; for the rest, which did not meet the standard were removed into the control group. All rats were randomly divided into 2,4,8,12,16 weeks(w). General situations, the urine, blood, biochemical indicators, Body weight(BW), KHI and the kidney damage score of all the rats were monitored at each time-point; kidney hypertrophy index(kidney hypertrophy index, KHI) of all the rats were detected punctually; the renal, retinal, myocardium and macrovascular morphology were examined, the kidney damage score 、myocardium damage grade 、RGC count were measured with hematoxylin-eosin(HE) stain. Analysis the correlation between the kidney injury indicators(24h-UP,KHI,Kidney damage score, s Cr) and retinopathy, myocadiopathy. Results: 1.The FPGs of DM groups were above 16.7 mmol/l, higher greatly the control groups, rats into the DM model was 100%, mortality rate of 0%; 2.The general situations(food intake and urine output), urine biochemistry indicator(24-UP) were significantly increased, though, the BW decreased continually, s Cr, BUN increased consistently; TP and ALB propagated gradually reduced. 3. pathology: The gradual emergence of kidney glomerular hypertrophy, mesangial matrix and mesangial cell proliferation, formation of focal sclerosis, tubular particles or degeneration, with cast formation; swelling of retinal ganglion cells, shedding less than the layers change thin, vacuolar degeneration, with formation of new blood vessels, myocardial hypertrophy, degeneration, necrosis, cardiac vascular dilatation and congestion, wall thickening, coronary artery endothelial cell swelling, loss, reduce plaque, vessel wall thickening, stenosis, occlusion, with inflammatory cell infiltration.4. KHI of DM group increased at first and then decreased,kidney damage score gradually increased; the RGC count of control group gradually increased, while DM subgroups’ decreased; the myocardial injury score of DM groups gradually increased. 5. the correlations between 24h-UP, KHI, kidney injury score, creatinine and retinopathy were significantly, kidney injury score, KHI and myocardial lesions were significantly correlated, while, 24h-UP, creatinine and cardiomyo- pathy did not relevant. Conclusion: 1. T1 DM rat model induced by STZ molded highly. 2. diabetes caused kidney, retina pathological changes typical time of 4-8 weeks, cardiac, macrovascular pathological changes typical for 12-16 weeks, the microvascular complications T1 DM earlier than macrovascular complications. 3.24h-UP could be used as indicators of early diagnosis of diabetic nephropathy. 4. DR and DN pathological changes were synchronous relationship, so DR could help to diagnose the early DN.