The Relationship Between Reactive Oxygen Species And Autophagy Within Peripheral Blood Mononuclear Cells In Premature Infants After Oxygen Exposed

Objective In order to explore the relationship between reactive oxygen species and autophagy within peripheral blood mononuclear cells in premature infants after oxygen exposed. Methods According to different oxygen concentrations, these preterm infants were divided into three groups, Fi O2<30% is low concentration oxygen group,Fi O2 between(30~40)% is middle concentration oxygen group,Fi O2>40% is high concentration oxygen group.Premature infants less than 32 weeks without oxygen is control group.After 48 h, the blood 3ml were taken via radial artery from each group,and used to be separated peripheral blood mononuclear cells(PBMC)and serum. Then detected intracellular reactive oxygen species(ROS) by confocal laser scanning microscopy, expression of autophagy by monodansylcadaverin combine confocal laser scanningmicroscope, malondialdehyde within serum by thiobarbituric acid colorimetric. Results After premature infants were exposed to oxygen, with the concentration of oxygen increased,ROS(0.053±0.0015),expression of autophagy(1.1955±0.0725) and MDA(15.6±2.01)gradually rised.Compared with the other three groups,ROS expression of autophagy and MDA in the high concentration oxygen group had increased significantly(p<0.05) in premature infants. Conclusions After premature infants were exposed to oxygen,high concentration of oxygen could induced increased reactive oxygen species(ROS) and autophagy occurs in PBMC.ROS could be used as signal molecules also increase cell autophagy reaction.