The Expression And The Significance Of CircRNA＿036766 In The Peripheral Blood Mononuclear Cells From IBD Patients

Objective:Inflammatory bowel disease(IBD)is a group of chronic,nonspecific inflammatory disease of the bowel,including Crohn’s disease(CD)and Ulcerative colitis(UC),and its pathogenesis has not yet been clarified.This study aims to screen differentially expressed circRNAs in peripheral blood mononuclear cell(PBMC)from CD patients by using circRNA microarray technology,and further verify the difference of circRNA expression level between IBD patients and healthy control(HC)and patient control(PC)PBMC,find a new biomarker for the diagnosis and evaluation of IBD,and explore its relationship with the pathogenesis of IBD.Methods:CircRNA microarray screening was performed in 5 active CD patients and 5healthy controls.Meanwhile,PBMCs were obtained from 60 CD patients,60 UC patients,30 healthy controls and 30 patients with irritable bowel syndrome(IBS).Then the expressions of circRNA＿036766 were validated by reverse transcription polymerase chain reaction(RT-PCR).Clinical indicators such as C-reactive protein(CRP)、erythrocyte sedimentation rate(ESR)and Fecal calcitron(FC)were collected.The diagnostic value of each circRNA was evaluated by receiver operating characteristic(ROC)curve.Results:A total of 384 differentially expressed circRNAs were detected by microarray screening,of which 155 were up-regulated and 229 were down-regulated.Further verification showed that compared with HCs and PCs,the relative expression levels of circRNA＿036766 was significantly up-regulated in IBD patients(P < 0.05).The area under the curve(AUC)of circRNA＿036766 for diagnosing CD and UC were0.6931 and 0.7794.In addition,circRNA＿036766 was positively correlated with CRP(r = 0.3619,P =0.0045),ESR(r = 0.3159,P =0.0139)and FC(r=0.3189,P=0.0130)in CD.Similarly,in UC patients,circRNA＿036766 was correlated with CRP(r = 0.6152,P< 0.05)、ESR(r = 0.4332,P=0.0005)and FC(r=0.7429,P< 0.05).Conclusion:CircRNA＿036766 in PBMCs of CD patients was significantly increased,and can be used as a potential biomarker for the diagnosis of CD.