Sodium Salicylate Effects On The Expression Of GABAa Receptor Subunits In Spiral Ganglion Neurons

Objective To investigate mRNA expression of GABAa receptor (GABAaR) subimits in the rat cochlear spiral ganglion neurons (SGN) and explore the effect of Sodium salicylate on the expression of GABAaR subunits.Methods The real-time fluorescent quantitative polymerase chain reaction, FQ-PCR)was used to detect mRNA expression of twelve GABAaR subunits in the newborn rat SGN and then investigate mRNA expression of GABAaR subunits after treatment with 5mmol/L SS for 15min,30min,60 min, 180 min and 360 min in the primary culture SGN.Results (1) GABAaR subunits of α1-6, β1-3, and γ1-3 were detected in the SGN, and the expression of GABAaR subunits were lower than those in the cerebral cortex. In the subunit a family of GABAaR, the expression rank was α2>α3/α5>α4>α1>α6, and the expression of α3 and as were no difference (P> 0.05). In the subunit β family, the expression rank was β3>β2>β1. In the subunit y family, the expression rank was yl>y2>y3. (2) The expression of all subunits of GABAa receptor were obviously fluctuated excepting subunit as after treatment with SS. At 15 min post-SS, α1,α2,β1 and γ1-3 were upregulated, and α3 was downregulated; At 30 min post-SS, α3, β1 and β3 were upregulated, and γ1 was downregulated; At 60 min post-SS,β2 was upregulated, and γ3 was downregulated; At 180 min post-SS, β1 and β2 were upregulated, and α3 and γ2 were downregulated; At 360 min post-SS, α1,α3, β2, β3 and γ1 were upregulated, and α2, α4 and β1 were downregulated.Conclusion The mRNA of GABAaR were expressed in the rat SGN, and the expression of GABAaR subunits were lower in SGN than the cerebral cortex. SS could alter the GABAaR expression quantity in rat SGN; Most of the subunits (α1-2, β1, γ1-3) expression were elevated obviously in the early post-SS (15 min), followed by a slight fluctuation.