Curcumin Inhibits Cell Growth And Invasion Through Up-regulation Of Mi R-7 In Pancreatic Cancer Cells

Background and Objective: Accumulating evidence has revealed that a natural compound curcumin has been shown to inhibit growth and metastasis in a variety of human cancers including pancreatic cancer(PC), and to be involved in regulating a variety of cellular signaling pathways. Studies have confirmed that mi RNAs have been demonstrated to play a crucial role in tumor cell proliferation, apoptosis, migration,invasion and metastasis. It can negatively regulates the expression of target genes, plays a tumor suppressor gene or a oncogene. In this study, we explore whether curcumin could inhibit cell growth, migration and invasion in pancreatic cancer cells.Methods:1.The effects of curcumin on As PC-1 and Bx PC-3 cells proliferation, apoptosis,invasion and migration were analyzed by MTT, Transwell migration and Annexin V-FITC/PI assay, respectively, in As PC-1 and Bx PC-3 cells lines. 2.The expression of mi R-7 in As PC-1 and Bx PC-3 cells was determined by real-time PCR assay after treatment with curcumin. 3.To detect the function of mi R-7 in cell proliferation,apoptosis and invasion, we transfected As PC-1 and Bx PC-3 cells with mi R-7 mimics,and combined with curcumin treatment. To further explore the effects of mi R-7 on cell proliferation, apoptosis and invasion, we also performed mi R-7 inhibition transfection and combination with curcumin treatment. 4.We further explored whether curcumin could inhibit the expression of SET8, one of mi R-7 targets, in pancreatic cancer cells through transfected with mi R-7 mimics and mi R-7 inhibition and combination with curcumin treatment.Result:1.Compared to control, curcumin treatment caused cells proliferation, invasion and migration inhibition in a dose-dependent manner in As PC-1 and Bx PC-3 cells(P<0.05).It revealed that curcumin can suppress cell growth, migration and invasion in As PC-1 and Bx PC-3 cells.2.Curcumin treatment induced cells apoptosis in a dose-dependent manner in As PC-1and Bx PC-3 cells. It revealed that curcumin can induce cell apoptosis in As PC-1 and Bx PC-3 cells.3.After curcumin treatment, curcumin increased mi R-7 expression in a dose-dependent manner in As PC-1 and Bx PC-3 cells.(P<0.05). It reavealed that curcumin can increased expression of mi R-7 in As PC-1 and Bx PC-3 cells.4.Mi R-7 mimics transfection experiment showed that compared with the control,curcumin group, mi R-7 mimics group caused cells proliferation and invasion inhibition,induced cells apoptosis. curcumin plus mi R-7 mimics group caused more significant ability than others(P<0.05). Simultaneously, mi R-7 inhibitor transfection experiment showed that compared with the control, mi R-7 inhibitor group decreased cells apoptosis,increased cells proliferation and invasion. These results showed that curcumin inhibited cells growth and invasion, induced cells apoptosis partly through up-regulation of mi R-7 expression in As PC-1 and Bx PC-3 cells..5.Mi R-7 mimics transfection showed that compared with the control, curcumin group,mi R-7 mimics group down-regulated the expression of SET8, and curcumin plus mi R-7mimics group caused more significant ability than others. Simultaneously, mi R-7inhibitor transfection experiment showed that mi R-7 inhibitor group up-regulated the expression of SET8. These results showed that curcumin down-regulated the expression of SET8 through up-regulation of mi R-7 expression in cells.Conclusion:1.Curcumin inhibited cell proliferation, migration and invasion and induced cell apoptosis in As PC-1 cells and Bx PC-3 cells.2.Curcumin inhibited cells growth, invasion, induced cells apoptosis partly and down-regulated the expression of SET8 through up-regulation of mi R-7 expression in As PC-1 cells and Bx PC-3 cells.