Immunolocalization Of CypA In Human Apical Periodontitis Lesions

AIM:CypA has multifunctions.It plays a key background role in various cell functions including protein folding,intracellular trafficking,signal transduction and transcription regulation. Recently,the potential roles of the CypA interaction have been identified in several diseases,such as rheumatoid inflammatory cardiomyopathy,diadetes,arthritis and tumor.Studies have demonstrated that CypA is involved in the pathogenesis of human periodontitis and in experimental rats periodontitis. Periodontosis and periapical periodontitis were both inflammation of jaw bone tissue and local connective tissue caused by bacteria,however,there is no data about CypA in periapical lesions showed before.The aim of this study was to examine the presence of CypA in human periapical lesions and its relationship with the clinical presentations of the lesions,which discuss the possible clinical roles of CypA in periapical lesion.METHODS:83 human apical periodontitis lesions(male 42, female 41) were consecutively obtained in Department of pathology after periapical surgery and tooth extraction in Oral and Maxillofacial Surgery of Anhui Medical University Affiliated Stomatological Hospital during a 7-month period(January to July 2014).The collection of patient information, including clinical manifestation and periapical film,the periapical radiography used in this experiment was bisecting angle technique.Two technicians blind of this experiment were told to measure the apical shadow and calculate the average diameter of the lesions.After pathological identification of the samples,according to the pathological type,the samples were divided into apical granuloma group and periapical cyst group; Then,according to the symptoms with pain, swelling and other obvious clinical symptoms in collected patient information,the samples were divided into symptomatic group and asymptomatic group. Six healthy dental pulp samples were obtained from a group of orthodontic tooth(male 3 cases, female 3 cases; aged 16-30 years) as the normal control group.The integrated optical density average value(A) of CypA and RANKL was measured by immunohistochemical analysis for all the samples.Differences in CypA and RANKL expression between different clinical presentations were subsequently analyzed by t’ test.RESULTS:1.CypA+cells and RANKL+cells were both detected in all periapical lesion tissues, and the expression of CypA was significantly higher in periapical lesions than normal tissues (P<0.05).2.Epithelial cells,endotheliai-like cells and lymphoid cells presented CypA positive immunostaining in the lesions.3.The expression of RANKL in cyst group were higher than granuloma group(Z=4.48, P<0.05),while the expression of CypA in cyst group were lower than granuloma group(Z=4.48, P<0.05).4.The expression of CypA in different clinical presentations was significant differences. In symptomatic samples,The integrated optical density average value(A) of CypA positive sites is 77.24(69.48-89.54),while the asymptomatic is 39.18(31.54-64.90).A P-value<0.05 was considered to be statistically significant-Positive correlations between the expression of CypA and the size of the lesions were observed(r=0.462, P<0.05); Positive correlations between the expression of RANKL and the size of the lesions were observed(r=0.417, P< 0.05).CONCLUSIONS:1, It’s the first time to demonstrate that CypA may be involved in the periapical inflammation response through the experiment of immunohistochemistry.2, There was a positive correlation between the expression of CypA associated with the osteoclastic factor-RANKL, indicating that CypA may be related with the bone resorption destruction.3, In this experiment,the expression of CypA in symptomatic group was higher than that in asymptomatic group, illustrate that CypA may be associated with the inflammation, progressThese results preliminarily showed the expression of CypA in periapical periodontitis,it’s beneficial to the further understanding of periapical disease pathogenesis.