| Objective:Schizophrenia is the illness occurs in approximately 1% of the global Population.Patients often require long-term medication treatment and also Place serious burden on families and society. Schizophrenia will also associated with a reduction in life expectancy, increased the rates of cardiovascular disease, diabetes and high blood pressure, increased greatly the incidence of diseases such as metabolic abnormalities. Recently years, the research found that the incidence of schizo Phrenia suffered type 2 diabetes is 3 ~ 4 times of ordinary People.One potential explanation for these clinical observation studies suggest that schizophrenia and correlation exists between schizophrenia drug resistance, diabetes. However, how to explain the mechanism of the correlation? Previous study of inflammatory cytokines in Patients with schizophrenia have increased. In addition, antipsychotics level changes of cytokines in Patients with schizophrenia. Inflammatory factors and the correlation of schizophrenia research has been reported,but its relationshipwith diabetes com Plications are not fully elucidated. Especially the expession of Pro-inflammatory factor and inhibit inflammation factor level and the correlation between schizo Phrenia and its complicated with diabetes is less clear, to be further discussed. This study intends to choose the healthy controls(NC), Patients with schizophrenia(SP), schizophrenia patients with diabetes(SPDM) mellitus as the research object, the detection of mononuclear cells, inflammatory factor IL–1β, TNFαand IL-10 m RNA expression level, and analyze its correlation with blood sugar and BMI. To explore its clinical significance, in order to make clear the relation between schizophrenia patients and inflammatory factor, delay the onset of diabetes, provide clinical basis for specification and anti-inflammatory drug treatment.Methods: 1 Objects of study: 1.1The Normal control group(normal control, NC) NC was selected from medical examination in a medical center of physical examination, a total of 16 cases, male in 7 cases, 9 cases of female, with mean age±standard deviation as 42.3±6.39, without diabetes, hypertension, hyperlipidemia, without atherosclerosis, and there is no history of chronic liver and kidney disease. The results of OGTT is normal. Healthy controls recruited did not have a significant Physical disabity or disease.None of them Presented with systemic illness or signs of fever at the time of sample collection. 1.2 schizophrenia group(SP) THE SP were the same Period of shijiazhuang hospital 8 severe Psychiatric inpatients with schizophrenia diagnosed by the DSM- IV-TR(American Psychiatric diagnostic manual version 4) and ICD- 10(the tenth edition of international classification of diseases), all patients diagnosis assessment conducted by a separate clinical physicians. None of the patients were acardia,cerebrovascular disease and acute and chronic infection, recently only treated with risperidone, PANSS 60 or more. Furthermore, the patients have no fever or other body disease too. 1.3 schizophrenia and the group of type 2 diabetes(SPDM) This group of patients are diagnosed with schizophrenia and patients with type 2 diabetes, is a diagnosis of schizophrenia Patients with DSM- IV-TR(American psychiatric diagnostic manual version 4) and ICD- 10(the tenth edition of international classification of diseases), all patients diagnosis assessment conducted by a separate clinical physicians. They were diagnosed diabetes by the world health organization(WHO) classification standard of diabetes diabetic grouping standard in 1999 of fit and in 1999. All the members to eliminate disease of heart head blood-vessel, and liver and kidney function is not complete, no acute and chronic infection, only recently taking risperidone, PANSS 60 or more. Samples, the patients have no fever or other body disease. This group of patients with a total of 29 cases, 13 cases were male, female 16 cases, mean age 48.11 ±15.698. 2 Experimental method 2.1 Preparation before sampling Before collecting the statistical Population data, such as height, weight, body mass index(BMI), the sex ratio, age range, SP and SPDM group still need statistical treatment time, daily dose drugs, PANSS, etc. 2.2 Routine biochemical examinations With automatic biochemical analyzer test routine biochemical indexes, such as fasting blood sugar, triglyceride, total cholesterol, etc., each test is the standard and quality control and product synchronized detection. 2.3 Mononuclear cell is extracted Extracting 2 ml whole blood, density gradient centrifugation separation of Peripheral blood mononuclear cells, extracting total RNA, use Real-time PCR detection of TNFα, IL-10 and IL-1βm RNA expression. 3.Statistical methods Using EXCEL and SPSS17.0 software for data statistics and finishing, the data to mean ±standard deviation, said compared using analysis of variance between groups, two further compare the LSD- t test, single factor with linear correlation analysis, P<0.05 for statistical significance.Results: 1 Routine data The FPG of healthy control(NC),Patients(SP),Patients with schizo Phrenia and diabetes(SPDM) was respectively 4.16±0.23mmol/L,4.99±0.72mmol/L,6.69±2.63mmol/L.The FPG are all higher in SP,SPDM than those in NC. Triglycerides of healthy control(NC), Patients(SP), Patients with schizophrenia and diabetes(SPDM) was respectively 1.16±0.19mmol/L,1.25±0.59 mmol/L,1.25±0.51 mmol/L. The Triglycerides are all higher in SP,SPDM than those in NC. 2 BMI levels in Patients with schizophrenia have altered. The levels of BMI in Patients with Schizophrenia(SP) group was(25.88 ± 3.18), suffering from schizophrenia and type 2 diabetes(SPDM) BMI levels(25.97 ± 3.13) compared to the healthy control group(NC) has significantly increased(22.14 ± 2.68) 3 The levels of TNF-α, IL-10,IL-1βm RNA expression in Mononuclear cells The results of Real-time PCR demonstrate the IL-10 m RNA expression of healthy controls,SP,SPDM was res Pectively 1.05±0.58,2.64±1.59,1.54±0.34. Compared with the normal control,that of SP was statistical significance The results of Real-time PCR demonstrate the IL-βm RNA expression of healthy controls,SP,SPDM was respectively 1.21±0.81,13.5±7.53,29.18±10.96. Com Pared with the normal control,that of SP and SPDM were statistical significance The results of Real-time PCR demonstrate the TNF-αm RNA expression of healthy controls, SP,SPDM was respectively 1.63±0.55,4.49±2.37,11.04±5.78. Compared with the normal control,that of SP and SPDM were statistical significance.Conclusion: 1 Suppression of inflammatory cytokine IL-10 m RNA expression levels were significantly increased in the SP group, suggesting that inflammation may be an important reason for the onset of schizophrenia. 2 TNF-α, the expression level of IL-1βm RNA in SPDM group increased, suggesting that it may be one of the reasons for diabetes in patients with schizophrenia. 3 BMI changes in patients with schizophrenia may be the cause of elevated inflammatory cytokines. 4 FPG Patients with schizophrenia associated with increased expression levels of proinflammatory cytokines IL-1βm RNA. |
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