The Application Of HE Staining And Immunohistochemical Staining In The Diagnosis Of Canine Breast Tumor

Canine breast tumor is one of common veterinary clinical tumor diseases with the incidence rate of 1.988‰, among which female cases account for more than 50% as shown by statistical analysis. It seriously affects the healthy development of the kennel industry and animal welfare improvement. The cause of canine mammary tumors, the nature of the tumor tissue as well as diagnostic and therapeutic methods are all similar to human breast tumor.Therefore, the dogs of naturally occurring mammary tumors have become the best animal model for the study of human breast tumors. With the improvement of living standards, pet owners put forward higher requirements for tumor treatment of qualitative analysis.This study collected pet hospital canine breast tumor cases as samples, with paraffin section as research method, with HE staining and immunohistochemical staining as research object. The clinical application value of them can be evaluated based on the observation of pathological sections, analysis of the result of immunohistochemical staining, comparison of accuracies of diagnosis on canine breast tumor by conventional dyeing technology of tumor tissue and immunohistochemical staining. The result shows that the HE staining can precisely tell whether the original tissue from which the pathological sections are sampled is from tumor tissue, normal mammary gland structure such as mammary gland flocculus, pipe and gland myoepithelial would be changed, connective tissue appeared hyperplasia and there are a lot of inflammatory cells in the organization. whilst observed from the result of immunohistochemical staining, prognosis judgment can be judged by the difference of positive expression rates occurred in benign breast tumor and malignant breast tumor where ER, PR and p53 specific immune responses with organization of canine breast tumor.