The Expression And Clinical Significance Of Bip In A Human Osteosarcoma Cell

Objective:The purpose of this research is to use different experimental methods to detect Bip presence in human osteosarcoma cell line Saos₂ cells, and further study its presence in 20 clinical osteosarcoma samples and compare the presence differences before chemotherapy and after chemotherapy and differences between transfer and non-transfer. This study will examine the relation(ship)s between Bip presence in osteosarcoma cells and osteosarcoma growth and transfer and impacts that chemotherapy have on its presence, so as to provide experimental and theoretical basis for osteosarcoma treatment and prognosis assessment.Methods:1 Immunofluorescence staining is used on the human osteosarcoma cell line Saos₂ experimental cells. The control group without immunofluorescence staining is compared to the experimental group by observing if there is Bip presence with the use of fluorescence microscopy.2 Take human osteosarcoma cell line Saos₂ cells and extract its total cellular RNA, conduct RT-PCR with the use of a RT-PCR kit, and observe if there is Bip mRNA presence.3 Examine 20 bone osteosarcoma clinical samples,6 of which are non-transfer,14 with transfer and 12 of which with preoperative chemotherapy and 8 without it. Make paraffin sections (slices) and perform immunofluorescence staining.Results1 There is Bip presence in human osteosarcoma cell line Saos₂ cells, mainly in the endoplasmic reticulum, the cell cytoplasm that was dyed green is positive. It's whole cytoplasm green when it's dyed darker and light foam-like cytoplasm green when it's dyed lighter.2 There is Bip mRNA presence in human osteosarcoma cell line Saos₂ cells.3 There is Bip presence in all samples. The intensity of osteosarcoma transfer group is stronger than the non-transfer one, the group with preoperative chemotherapy is stronger than the one without it. There are significant differences in presence quantities between transfer and non-transfer osteosarcoma groups and preoperative chemotherapy and non-preoperative chemotherapy groups.Conclusion:This research shows that Bip can stimulate tumor growth, survival and metastasis, it has respective reactions to chemotherapy treatment. Therefore, Bip can be a molecular targeting marker for osteosarcoma growth and metastasis and response to therapy and can be applied to clinical work as biomarker for judging osteosarcoma development and prognosis. Lowering Bip presence by genes may suppress the growth of osteosarcoma cells and increase their sensitivity to chemotherapeutic drugs, control their transfer and improve prognosis. Suppression of Bip presence in the treatment of osteosarcoma may represent a new eradication of residual method. Therefore, the use of Bip as a molecular target or medium for osteosarcoma gene therapy has broad application prospects.