| Objective Intrauterine adhesions(IUA) is one of the common gynecological diseases affecting the health of childbearing women currently, has become one of the common causes of female secondary infertility. The most common cause of IUA is intrauterine operations, the formation mechanism of IUA is unclear still, there is no ideal effective treatment, estrogen after intervention for the prevention of abortion IUA formation remains controversial, its treatment and pathogenesis become major problems plagued the clinician. The prevention of the occurrence of IUA after intrauterine operations, especially after uterine curettage operations have a great significance. The prevention of IUA and the recurrence of IUA postoperative have become the academic research focus. Restricted by the ethics, a stable ideal experimental animal model for in-depth study ofthe formation mechanism of intrauterine adhesions and prevention methods is urgent. Selecting a appropriate animal as the intrauterine adhesions model, can provide a good platform for the IUA related research. This study was expected to establish a stable rat model of intrauterine adhesions, and making a preliminary study of intrauterine adhesions formation by estrogen intervention in this model.Methods(1)The establishment o f the rat intrauterine adhesions model: Selecting estrus Wistar rats by general vaginal smear make a analog curettage use self-made miniature curette. Experimental group: the left uterine makes a trauma treatment, and the right side of the uterus does not deal. Depending on the severity of scraping, divided into mild injuries and severe injuries. The normal rats took as control group. The two groups of uterus specimens were collected at 0 h, 24 h, 72 h, 7 d. Paraffin-embedded sections underwent HE, Masson staining and the adhesion factors of immunohistochemistry observe the expression in the endometrium.(2) Estrogen in preventing intrauterine adhesions: Experimental control group:choosing estrus Wistar rats by regular vaginal smear, using homemade miniature curette scraped the uterine seriously. The left uterine made a trauma treatment, the right side of the uterus does not deal, and two ovaries were kicked out. Experimental group was given immediately 17β-estradiol subcutaneously, every morning at 9:00 h 17β- estradiol was administered subcutaneously. The normal rats took as control group. Collect uterine specimens of three groups were 24 h, 72 h, 7 d receptively. Paraffin-embedded sections underwent HE, Masson staining and the TGF-β1and α-SMA factors of immunohistochemistry observe the expression in the endometrium.Results(1) In this study, using micro- curette simulation the clinical endometrial curettage were scraping on rats, divided into mild and severe damage to the endometrium. From HE, Masson staining morphology and b FGF, TGF-β1 and α-SMAimmunohistochemistry, endometrial injury in rats and ending with the outcome of intrauterine adhesions after curettage was similar to the human pathological features prognosis, TGF- β1 and α-SMA of the severe injury group has more significantly different at each time point than the mild injury’s, suggesting that the two factors participate the formation of intrauterine adhesions.(2)The rats after endometrial injury were given 1μg estrogen intervention, compared with the control group, the histology, Masson staining showed that endometrial fibrosis mitigation; TGF-β1 and α-SMA expression significantly reduced(p <0.05).Conclusions(1) In our study, using homemade curette simulation curettage method succeeded in establishing a stable mechanical damage rat model of intrauterine adhesions.(2) the use of estrogen in preventing the formation of intrauterine adhesions in rats, suggesting that 1μg estrogen can reduce the formation of fibrosis. |
PDF Full Text Request