The Influence Of Different Kinds Of Collutory On Bacteria Amount In The Mouth

Objective: This test used patients who needed dental treatment as the research objects.Before treatment,patients rinsed the mouth using respectively compound chlorhexidine gargle,Listerine gargle, Colgate teeth gargle and clear water.Detected pathogen changes before and after the mouthwash,compared the antibacterial effect of mouthwash on the intra oral pathogen, thus provided the theory basis on how to choose mouthwash for patients needing dental treatment, in order to prevent or reduce the risk of cross infection.Methods: Chose 80 patients who had teeth defect needing treatment and visited Baoding First Central Hospital Stomatology Clinic From January 2014,to June 2014.Among them,there were 42 males and 38 females,aging from 25 to 53, the average age was about 34 years old. Inclusion criteria: healthy body, no history of drug allergy, no antibiotics within one month, women without pregnancy and not in lactation, good oral health,needing crown restoration. Patient informed consent.The patients were divided into four groups according to random number table in clinic order, 20 cases in each group.Group A: compound chlorhexidine gargle;Group B: Colgate teeth gargle for 1 minute;Group C: Listerine gargle for 1 minute;Group D: clear water gargle for 1 minute;Took bacterium randomly and symmetrically at left and right sides of molar level of palatal mucosa with 1.0 ㎝2 size filter after sterilization respectively before and after mouthwashing, bacteria taking area was about 1.0 ㎝2.Took out the filter with sterile forceps after 30 seconds to the centrifuge tube containing a 1.0 ml PBS solution. After the shocking and mixing by the shock machine, diluted 10 times, took 10 ul solution inoculating on the agar plates at a 37 incubator, cultured for 48 hours after ℃the colony count(CFU/ml), and calculated the total bacteria amount.Results:1 There were no statistical differences between patients’ sex ratioand age in each group(P>0.05).2 There were no statistical differences on the bacteria amount before mouserinsing on four groups(P>0.05).3 Using paired test within groups,the bacteria amount before and after the mouthwash were statistically significant on all groups(P<0.05).4 The differences before and after mouthwash between the control group(group D) and the experimental groups(group A, B, C) were statistically significant(P<0.05).5 The difference before and after mouthwash between the compound chlorhexidine gargle group and the Listerine gargle group was statistically significant(P<0.05).6 The difference before and after mouthwash between the compound chlorhexidine gargle group and the Colgate teeth gargle group was statistically significant(P<0.05).7 There was no statistical significance on the difference before and after mouthwash between the Listerine gargle group and the Colgate teeth gargle group(P>0.05).Conclusion:1 Using mouthwash before oral treatment can effectively reduce the number of bacteria in the mouth and the risk of cross infection.2 The effect is better on reducing the number of bacteria in the mouth with collutory than water.3 The compound chlorhexidine gargle is better in removing bacteria in the mouth than the Listerine gargle and the Colgate teeth gargle.4 The Listerine gargle and the Colgate teeth gargle have the same effect on removal of oral bacteria.