| Breast cancer is the most common malignancy of women, the incidence is gradually getting younger and younger, the mortality rate is increasing year by year, some big cities women reported breast cancer accounted for first. The pathogenesis of breast cancer is very complex, at present, more unified understanding of breast cancer occurrence, development and prognosis of a variety of factors, complex pathological process of joint participation in different genes. With the rapid development of molecular biology and biotechnology emerging high-tech, in addition to major surgery, hormonal therapy, radiotherapy, chemotherapy also improve the survival rate, improve the patient’s quality of life aspect plays an important role, With the continuous improvement of prevention and treatment, hormone therapy, and biological therapy are increasingly the subject of attention, and related genes of breast cancer research and related drugs these treatments provide a reliable theoretical basis. Currently, many experts believe that the detection of breast cancer markers should be taken seriously, it can reflect the biological characteristics of tumor cells, to provide a reliable reference for clinical diagnosis and guide therapy, predict the development and prognosis of tumors. ﹝ 15 ﹞Tumor cell apoptosis and proliferation of imbalance and abnormal, leading to the occurrence and development of breast cancer. Caspase That cysteine protease, is present in the cytoplasm, the family of Caspase-3 play in the process of apoptosis in an irreplaceable role, DIAPH-3 protein belongs Formin protein family, constitute the cytoskeleton actin-binding protein, protein filaments extending aspects of their movement played an important role in actin monomers nucleation and muscle. Studies have found that in breast cancer cells, DIAPH-3 protein was down-regulated or absent, to build astable silence DIAPH-3 expression vector, by silencing DIAPH-3, found DIAPH-3 after disturbance, unstable microtubule protein production, cell invasion and metastasis than silence before DIAPH-3 significantly enhanced.Objective: To detect caspase-3 and DIAPH-3 protein in invasive breast cancer and adjacent cancer tissues, to explore the relationship between patient age, tumor size, TMN staging and clinical pathology, research caspase-3 and DIAPH The correlation between-3 and to further explore the relationship between breast cancer, metastasis and prognosis.Methods: Shijiazhuang City, the first hospital to collect breast cancer and 54 cases of adjacent tissues, tumors and cancerous tissue between 2009-2010 invasive breast cancer after resection surgery were formalin-fixed and paraffin-embedded, all patients the former were not received formal radiotherapy, chemotherapy and endocrine therapy. According to TNM(UICC UICC) clinical staging. All patients were female, were pathologically confirmed invasive breast cancer, aged 32-83 years old, with a median age of 57 years old, I 26 cases of patients, II in 16 cases, III of its 12 cases. Detect the expression of DIAPH-3 and Caspase3 protein by immunohistochemistry Eli Vision law, to investigate the expression and clinicopathological features of invasive breast cancer, age, tumor size, lymph node metastasis, clinical stage, tumor vasculature relevance; Kaplan-Meier Survival curves were analyzed by univariate survival analysis; and the use of COX regression model was used for multivariate survival analysis; explore relationships and Caspase3 DIAPH-3 expression with invasive breast cancer prognosis.Result:1 Caspase-3 positive expression rate of normal breast tissue and breast cancer tissues were respectively 86.67%, 42.15%, the statistical analysis, Caspase-3 positive expression differences in normal breast tissue, breast cancer was statistically significance(P<0.05), DIAPH-3 expression rate in normal breast tissue and breast cancer tissues were respectively 86.67%, 36.36%, the statistical analysis, DIAPH-3 in normal breast tissue, breast cancer positive The expression of pairwise comparisons difference wasstatistically significant(P <0.05).Correlation between expression and clinical pathology 2 Caspase-3’s The total number of lymph node metastasis Caspase press, with or without vascular invasion, ER, PR, Her-2, P53 negative and positive group, analyze the expression of Caspase in each group is statistically significant. Caspase press without lymph node metastasis, lymph node metastasis 1-3, three more groups of lymph node metastasis, the positive expression rates were 70%, 66.2%, 61.9%, among the groups was statistically significant(P <0.05) difference indicates Caspase-3 expression and lymph node metastasis. Caspase-3 positive expression in vascular tumor thrombus group was 71.2%, the positive expression in the absence of vascular invasion group was 77.3%, the difference between the two groups was statistically significant(P<0.05); Caspase-3 in the ER The expression between PR, Her-2-positive and negative groups was not statistically significant(P<0.05) :; Caspase-3 in the positive expression of P53 positive group was 77.3%, in the positive expression of P53 was negative 71.9% The difference was statistically significant(P<0.05).Correlation 3 Caspase-3 expression and clinical casesCaspase-3 by age, clinical stage and tumor size are grouped analyze whether a statistically significant groups to see expressed. Expression of Caspase-3 expression in the older than 50 age group was 70.8% less than the positive rate was 50-year-old group, 73.3% between the two groups showed no significant difference(P>0.05), that is, of Caspase-3 No direct relationship between the prevalence of age. Caspase-3 in clinical stage I, II, III group of positive expression rate was 73.1 percent, respectively, 75.0%, 75.0%, the difference between groups was not statistically significant(P>0.05). The positive rate of Caspase-3 in tumor diameter less than 2.5cm group and greater than 2.5cm group was respectively 78.1%, 68.2%, the difference between the two groups was not statistically significant(P>0.05), namely Caspase-3 expression and tumor size no direct relationship.Correlation between expression and clinical pathology 4 DIAPH-3’sDIAPH-3 by the total number of metastatic lymph nodes, with or withoutvascular invasion, ER, PR, Her-2, P53 grouping analyze whether statistically significant expression in each group. DIAPH-3 under the no lymph node metastasis, lymph node metastasis 1-3, three more groups of lymph node metastasis, the positive expression rates were 45%, 61.9%, 69.2%, the difference between the groups was statistically significant(P<0.05) that DIAPH-3 expression and lymph node metastasis. DIAPH-3 by having vascular tumor thrombus and no vascular invasion group, the positive expression rate of 50% and 63.6%, the difference between the two groups was statistically significant(P<0.05); DIAPH-3 in ER-positive group The positive rate of 66.7%, the positive rate was negative in 33.3% between the two groups showed no significant difference(P>0.05); DIAPH-3 expression in PR positive group was 66.8% in the negative group The positive rate was 40.9%, the difference between the two groups was not statistically significant(P> 0.05); DIAPH-3 expression of Her-2 positive group at 66.7%, the positive-negative group at 79.2% between the two groups showed no significant difference(P>0.05). DIAPH-3 positive expression rate of P53 negative 50% in the rate of positive expression group of 68.2% between the two groups showed no significant difference(P>0.05).Correlation 5 DIAPH-3 expression and clinical casesDIAPH-3 by the prevalence of age, clinical stage, tumor size grouping analyze whether the expression was statistically significant in each group. DIAPH-3 in the diseased older than 50 years and less than the expression of 50-year-old group was 45.8% and 66.7%, respectively, between the two groups showed no significant difference(P>0.05), namely DIAPH-3 expression and sick Age is no direct relationship. DIAPH-3 clinical I, II, III group of positive expression rate was 46.2 percent, respectively, 68.8% and 66.7%, the difference between groups was not statistically significant(P> 0.05). DIAPH-3 expression of tumor diameter greater than 2.5cm in group was 40.9%, the positive expression group of less than 2.5cm in 68.6% between the two groups showed no significant difference(P>0.05), namely DIAPH-3’s expression and tumor diameter no direct relationship.Correlation 6 Caspase-3 and DIAPH-3’sDIAPH-3 positive expression was 23% in Caspase-3 positive and negative group, 7%, DIAPH-3 on Caspase-3 positive and negative negative expression group was 15%, 9%, respectively, the difference was not statistical significance.Conclusion:1 Caspase-3 and DIAPH-3 in invasive breast cancer are downregulated. 2 Caspase-3 protein expression and tumor size, clinical stage, age, ER, PR, etc. were not correlated with lymph node metastasis and vascular invasion related to its low expression in breast cancer and breast cancer may be prompted, development process, there is inhibition of apoptosis, abnormal cell proliferation and tumor metastasis. It may not be an independent and effective indicator to predict metastasis and prognosis. 3 DIAPH-3 expression and the number of lymph node metastasis in invasive breast carcinoma, and PR, Her-2, P53 has nothing to do with tumor invasion and metastasis. |
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