Impact Of Early Oifactory Deprivation On Subunit In Hippocampus Of SD Rats

Objective: The purpose Olfactory system is one of the most primitive human sensory system, the most important system, smell is a feeling that people and animals through the sense of smell to distinguish odors, racial discrimination, and other similar liaison; animal feeding, recognize objects, emotional communication and one important way of courtship, the hippocampus has the ability to manage learning and memory, and learning and memory are higher mammals unique to higher nervous activity. Hippocampus and statements not only remember, but also to spacial memory is also closely linked to this article on the olfactory deprivation on hippocampal gyrus, early brain development due to central nervous system damage caused by the slow development of olfactory learning and memory and behavioral abnormalities possible explore the role.Method:SD rats were 36(The rats were provided by the Experimental Animal Center of the University School of Medicine), weighing between 75-115 g, average weight 100 ± 12 g, these rats were fed in the cage, keeping in room temperature between 23 ℃-25 ℃, using a random number table of the study subjects were divided into control group, olfactory deprivation groups of 18.Results:1 The general morphologyStudy found that mice exhibit olfactory deprivation group at this time quiet, small body, unresponsive and no profit and avoid loss of consciousness, its weighing found that the average body weight of 67.16±4.98 g, the control group was active, normal body development responsive, presence awareness while avoiding disadvantages, weighed after its discovery, the average weight of 89.06±5.11 g, to compare the two groups showed no difference in body weight, and the difference was statistically significant(t=5.11, P= 0.03). 2 Nissl stainingThe number of blank control group, the hippocampal dentate gyrus neurons(1048.1), the number of olfactory deprivation group of neurons in the dentate gyrus of the hippocampus(947.9) The number of neurons in the hippocampal dentate gyrus, difference between the two groups, the difference was statistically significant(P<0.05). 3 MORRIS water maze test results 3.1 navigation testStudy found that the control group, the escape latency was 4.9±1.2 hours, and the olfactory deprivation group escape latency was 8.8±1.1 hours, and the two groups were compared, the results found that the differences between the two groups, the difference was statistically significant(t=5.88, P=0.02). 3.2 Space exploration experimentStudy found that these two groups of patients was found in space exploration: space exploration control group compared with the experimental results increased olfactory deprivation group, two groups of patients was compared with that there are differences, the difference was statistically significant(P<0.05). 4 The test results immunohistochemistry 4.1 δ subunit immunohistochemical resultsImmunohistochemical staining showed that the control group and the group of olfactory deprivation Neu N positive cells were mainly distributed in Tier III and Tier II, Tier I have two in a small amount of the distribution, at different points in time its different Neu N positive cells, blank control group and olfactory deprivation group differences subunit immunohistochemical results, the difference was statistically significant(P<0.05). 4.2 Cumulative absorbance analysis resultsCumulative absorbance method using(IOD) α5 subunit immunohis- tochemical analysis results, the researchers found that study found that the control group and the olfactory deprivation group differences in subunit immunohistochemical results, the difference was statistically significant(P<0.05). 5 Quantitative PCR results 5.1 PCR amplification Gel ImagingPrior to the use of q-PCR do RT-PCR, the experimental results showed that the control group and the experimental results of olfactory deprivation group distributed as follows, there are differences between the two groups, the difference was statistically significant. 5.2 δ subunit quantitative PCRExperimental process real-time PCR and melting curve δ subunit of GAPDH is generally a single peak, if the PCR melting curve showed a single peak, said both are specific expression, and for the amplification curve is good. Study found, δ subunit quantitative PCR results of two groups of patients in the following figure: control group and olfactory deprivation group differences in δ subunit quantitative PCR results, and the difference was statistically significant(P <0.05). 5.3 α5 subunit quantitative PCRPrevious studies have found that real-time quantitative PCR α5 subunits and the melting curve of GAPDH as a single peak, both of which are specifically expressed during the experiment, and the amplification curve is good. And good for the amplification curve. Study found, δ subunit quantitative PCR results of two groups of patients in the following figure: control group and olfactory deprivation group differences in δ subunit quantitative PCR results, and the difference was statistically significant(P<0.05).Conclusions:1 The basic form of olfactory deprivation affects function in rats.2 olfactory deprivation affects the differentiation of neurons in rats.3 olfactory deprivation affects the distribution and expression in the hippocampus Neun.4 olfactory deprivation affects the distribution and expression in the hippocampus and δ subunits α5 GABAA receptor subunit.