Effects Of Rem Sleep Deprivation On CRF And CRF Receptors MRNA In Depressive Rats' Medulla Oblongata,hippocampus And Hypothalamus Treated With Chronic Stress

AIM: To observe the changes of the depressive rats'self behavior,visceral sensitivity,CRF and its receptors mRNA in medulla oblongata,hippocampus and hypothalamus induced by sleep deprivation. The depressive rats were treated with chronic mild unpredicted stresses.METHOD: Wister rats were randomly divided into normal control group, depression group, large platform group and a small platform group. The small platform group is the sleep-deprivation group. Use the open-field score method to determine if the model of depression is successful and determine the self behavior and activities, Use AWR score method to determine changes in visceral sensitivity, use Real-time PCR to detect the mRNA of CRF and its receptors in the hypothalamus, hippocampus and medulla oblongata.RESEALT: Compared with the control group, The body weight,self-behavior activity and visceral sensitivity of the depressive rats were significantly different, P <0.05., In the Depression group, weight lost, self-behavior activity decreased, intestinal sensitivity increased. Sleep deprivation can increase self-behavior in rats, reduce the sensitivity of the intestinal tract, P <0.05. No effect on body weight. The CRF and receptor CRF-R1 mRNA levers in the central of the depressive rats were higher, such as hypothalamus,hippocampus and medulla oblongata, compared with the control group, there were significant difference. There were no changes in CRF-R2. Sleep deprivation can reduce the CRF and CRF-R1 mRNA levels in these parts of depressive rats, but have no impact on CRF-R2.CONCLUSION: Sleep deprivation can significantly improve the self behavior and visceral sensitivity. of depressive rat model by regulating the lever of CRF and its receptors...