Effects Of Formaldehyde Inhalation On Neurons Of Olfactory Bulb And Hippocampus In Rats

In order to study the effects of formaldehyde inhalation on the morphological damage as well Glu, GABA and NOS content in olfactory bulb and hippocampus of SD rats, also to further explore the effects of formaldehyde on learning and memory. Twenty SD rats were divided into two groups randomly, ten mice each. The normal group were inhaled with outdoor clear air, while the experimental group were exposed to the formaldehyde toxical air(12.5mg/m~3). Rats were sacrificed one week after treatment. Frozen sections of olfactory bulb and hippocampus were made, then Nissl-staining, immunohistochemistry and western blot were performed to tested the variation of Glu, GABA and NOS. Compared to the normal group, there was a significant confusion and shrink of neuron morphology in formaldehyde inhaled one. What's more, the number and staining intensity of positive cells as well protein expression of Glu and NOS were lower in formaldehyde group , but Protein content of GABA was Realized for the decline in treated group. Formaldehyde inhalation had a severe morphological damage in olfactory bulb and hippocampus of SD rats, which may further lesion memory and learning through the reduction of Glu, GABA and NOS expression.