Study On The Correlation Between Plasma Wnt5a And The Severity Of Coronary Heart Disease

Objectives:Comparing the concentrations of serum wnt5 a under different number of branches,different degrees of stenosis and different clinical symptoms in patients with coronary heart disease, and analysying the correlation with other risk factors of coronary heart disease to explore the function of wnt5 a as an inflammatory secreted protein in the development of coronary heart disease and its severity.Methods:A total of 125 patient was collected Selectively from the First Affiliated Hospital of Bengbu Medical College during April 2014 to December 2014; Of which 100 cases were coronary heart disease group, the others was the control group. General information and detailed clinical data on the patients in each group were collected;and combined with clinical symptoms and related inspection, number of diseased vessels, Gensini quantitative score, history of diabetes CHD, CHD groups further was subdivided: stable angina, unstable angina, myocardial infarction; single-vessel disease, double vessel disease and multivessel disease; mild, moderate and Severe; CHD&diabetes 、 CHD & non-diabetes.wnt5 a serum concentration was measured by enzyme-linked immunosorbent assay(ELISA), and serum hs-CRP concentration was measured by immune scatter turbidimetry.The differences of them were analyzed between the various subgroups. The relevance between serum wnt5 a and various coronary risk factors as well as of Gensini score was decided by correlation analysis. Acute coronary syndrome is a variable, various coronary risk factors as independent variables, using multivariate binary logistic regression,we explore whether or not wnt5 a was an independent risk factor. Statistical data was analysised by SPASS19 software, P <0.05 was considered statistically significant.Results: 1.Independent samples t test showed: fasting blood glucose(FBS), high-sensitivity C- reactive protein(Hs-CRP), Wnt5 a in CHD group were significantly higher than the control group, and high-density lipoprotein(HDLC) significantly lower than the control group(P <0.05). the number of patients with hypertension and diabetes in CHD group was higher(P <0.05), the other indexes didnt(P >0.05).2.ANOVA showed:the level of plasma Hs-CRP plasma between the control group, stable angina, unstable angina, myocardial infarction((1.01 ± 1.08, 1.10 ± 0.78,1.56 ± 1.09, 2.08 ± 1.08) had statistically significant differences. Pairwise comparisons:Myocardial infarction was significantly higher than the other three groups(2.20 ± 1.15 VS 1.01 ± 1.08, 1.17 ± 0.78, 1.46 ± 0.98) and the comparisons with any of the above groups(P <0.05).Unstable angina and stable group were higher than the control group(P <0.05).ANOVA showed: the level of plasma Wnt5 a between the control group, stable angina, unstable angina, myocardial infarction(432.32±77.69,505.97±53.80,553.54±90.31,603.88±86.80) had statistically significant differences. Pairwise comparisons:the comparisons with any of the above groups(P <0.05).3. Independent samples t test showed :Plasma wnt5 a in diabetes with coronary heart disease was higher than non-diabetic patients with coronary heart disease group(577.18 ± 88.164,540.38 ± 86.26). Chi-square test shows the former had a higher proportion of non-single-vessel disease and the difference was statistically significant(P <0.01), contingency coefficient=0.27.4. ANOVA showed: the level of plasma Wnt5 a between the control group, single-vessel disease, double vessel disease and multivessel disease group(432.32 ± 77.69, 517.09 ± 67.17, 541.31 ± 79.86, 620.41 ± 84.94) had statistically significant differences(P<0.01). Pairwise comparisons: Single vessel disease group and double-vessel group, s level was higher than the control group(P <0.05); Multivessel disease group was higher than the control group(P <0.05); multivessel disease was higher than that of single-vessel disease and double-vessel disease group(P <0.05), double vessel disease group was higher than that of single-vessel disease group(P> 0.05).ANOVA showed: the level of plasma Hs-CRP between the control group, single-vessel disease, double vessel disease and multivessel disease group(1.01±1.07,1.09±0.91,1.63±1.02,2.12±1.14)had statistically significant differences(P <0.01). Pairwise comparisons: Multivessel disease group(2.12 ± 1.14) is higher than the any group(P <0.05), the other pairwise comparison(P> 0.05).5. ANOVA showed : the level of plasma Wnt5 a nt5a between plasma, mild, moderate and Severe(432.32 ± 77.69, 521.94 ± 57.30,554.43 ± 79.31, 670.11 ± 82.15) had statistically significant differences(P <0.01). Pairwise comparisons:Severe disease group was higher than any of other group(P <0.05), mild and moderate disease group was higher than the control group(P <0.05), the mild disease group was lower than the moderate disease group(P> 0.05).6. Bivariate correlation analysis and partial correlation analysis showed: the level of plasma wnt5 a and Hs-CRP(r = 0.48, r = 0.346), Gensini score(r = 0.51, r = 0.312), fasting plasma glucose(r = 0.398, r = 0.294) were positively correlated(P <0.05).7. Multivariate binary logistic regression analysis showed: ACS and wnt5 a, Hs-CRP was positively correlated(P <0.05), and high-density lipoprotein was negatively correlated(P <0.05)Conclusions:1. With the onset of coronary artery disease plasma and its severity,wnt5 a was increased, so to some extent,it can indirectly reflect the severity of coronary artery disease. And it maybe as a risk factor for ACS, suggesting that it could become clinical predictor of patients with the risk of ACS.2. the level of plasma wnt5 a and lipid metabolism had no correlation.3. the level of plasma wnt5 a and Hs-CRP(r = 0.48, r = 0.346) were positively correlated,which showed it maybe involved in the formation and development of atherosclerosis as a secreted inflammatory protein.