| Objective: To investigate the role of mi R-23 a, mi R-24-2, mi R-27 a and mi R-223 in the pathogenesis of primary gouty arthritis.Methods:(1) Real-time quantitative polymerase chain reaction(RT-q PCR Taq Man) was used to detecting the expressions of mi R-23 a, mi R-24-2, mi R-27 a and mi R-223 in peripheral blood mononuclear cells(PBMCs) in AG, IG and HC; Clinical and laboratory parameters were collected in all the participants.(2) The PBMCs of HC were stimulated 12 hours with 100μg/ml monosodium urate(MSU), Phosphate Buffered Saline(PBS).(3) The expressions of mi R-23 a, mi R-24-2, mi R-27 a, mi R-223 and NLRP3 were examined with RT-q PCR in PBMCs.(4) The levels of IL-1β in the supernatant were measured with enzyme linked immunosorbent assay(ELISA).(5) Data were analyzed using SPSS17.0 statistical software, Wilcoxon rank sum test, t test and Spearman’s correlations analysis were used for statistical analysis.Results:(1) The expressions of mi R-23 a, mi R-24-2, mi R-27 a and mi R-223 in AG(0.006±0.023, 0.986±1.313, 0.086±0.288, 8.665±17.396) and IG(0.004±0.0251, 0.277±2.087, 0.0905±1.754, 7.621±16.531) were significantly lower than HC(0.180±0.629, 4.595±4.273, 0.489±2.474, 25.992±75.905). There are significant differences in three groups(Z=34.690, 11.334, 9.762, 11.387; P<0.01, respectively); The levels of mi R-223 and mi R-27 a were lower in AG than IG(P<0.01,respectively), but mi R-23 a, mi R-24-2 were lower in AG than IG,and there are no significant(P>0.05, respectively).(2) The relative expressions of mi R-23 a, mi R-24-2, mi R-27 a and mi R-223 in MSU stimulated group(0.0026±0.0004, 1.2665±0.2112, 0.0434±0.0028, 0.335±0.201) were reduced significantly than that in the PBS control group(0.0168±0.0056, 1.6256±0.2634, 0.1644±0.0319, 1.051±0.236)(T=-5.04, P<0.01; T=-2.61, P<0.05, T=-7.55,(P<0.01; T=-5.164, P<0.01).(3) The m RNA levels of NLRP3 in PBMCs and IL-1β in the supernatant after MSU stimulation(5.231±0.432, 86.235±4.586)were significantly higher than that in control group(1.156±0.448, 12.587±6.342) with a statistically significant difference(t=14.640, 21.042; P<0.01, respectively).(4)The expressions of mi R-23 a, mi R-24-2, mi R-27 a was correlated positively(r=0.548, 0.690, 0.523; P<0.01, respectively) in PBMCs of GA; In the GA patients, the levels of mi R-24-2 and mi R-223 was correlated positively with total cholesterol(r=0.46, 0.45, P<0.05, respectively), the level of mi R-24-2 was correlated positively with apolipoprotein B100(r=0.44,P<0.05).Conclusion: The expressions of mi R-23 a, mi R-24-2, mi R-27 a and mi R-223 in AG are significantly lower than that in HC and inflammatory environment in vitro; The lower expression of mi R-223 attenuated its inhibitory effect to NLRP3 inflammasome prompting IL-1β release, It suggested that they may participated in the regulation of inflammatory immune response in development of goutin; addition to mi R-24-2,mi R-223 may also be involved in regulating the lipid metabolism of gout. |
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