Effects Of Endoplasmic Reticulum Stress On The Invasion And Migration In Gastric Cancer Cell

Background and ObjectiveA number of factors, such as hypoxia, nutritional deficiency, oxidative stress, calcium metabolism disorders and defects in protein expression can cause ER stress. ER stress could trigger a series of cyto-protective responses, this process is termed as the unfolded protein response(UPR). Recently, it has been shown that ER stress is commonly present in various tumor tissues and could trigger a range of cyto-protective responses, which contributes to the metastasis of tumor cells. The relationship between ER stress and the invasion and migration in gastric cancer remains unclear yet. In this study,gastric cancer cells SGC7901 and BGC823 were treated with tunicamycin(TM) and the effects of ER stress on the invasion and migration was investigated. Methods:1.Establish ERS cell model SGC7901 and BGC823 cells were treated with 5μg/ml TM for 0h, 12 h, 24 h and 36 h, respectively. Expression of GRP78 at protein level was detected at each time point using Western blot.2.The wound healing assay and Transwell assay SGC7901 and BGC823 cells were treated with 5μg/ml TM for 0h、12h、24h and 36 h, respectively. The wound healing ability was observed by wound healing assay. The migrating and invasive abilities were examined using transwell assay.3.Detect changes of metastasis-related protein under ERS SGC7901 and BGC823 cells were treated with 5μg/ml TM for 0h、12h、24h and 36 h, respectively. Expression of MMP-9 and VEGF at protein level was detected at each time point using Western blot.4.Statistical analysis All data were showed as mean standard deviation(X±S) and analyzed with SPSS17.0 statistical software. P <0.05 was considered as statistically significant. Results1.TM can induce ERS in SGC7901 and BGC823 cells Compared with the GRP78 protein expressin in group 0h,that in SGC7901 and BGC823 cells treated with TM for 12h、24h and 36 h was markedly increased of time-dependent existence, respectively(P<0.05).2. The effects on the invasion and migration of gastric cancer cells by ER stress Compared with the abilities of invasion and migration in group 0h, those in SGC7901 and BGC823 cells treated with TM for 12h、24h and 36 h was markedly increased of time-dependent existence, respectively(P<0.05).3.The changes of metastasis-related protein under ERS Compared with the MMP-9 protein expressin in group 0h,that in SGC7901 and BGC823 cells treated with TM for 12h、24h and 36 h was markedly increased of time-dependent existence, respectively(P<0.05).Compared with the VEGF protein expressin in group 0h,that in SGC7901 and BGC823 cells treated with TM for 12h、24h was markedly increased of time-dependent existence, respectively(P<0.05). Compared with the VEGF protein expressin in group 24 h, that in group 36 h was decreased(P<0.05). Conclusion:ERstress upregulates MMP-9and VEGF in gastric cancer cells, promoting gastric cancer cell migration and invasion.