| Backgrounds: FOXP3 is an important transcription factor for maintaining the function and normal development of regulatory T(Treg)cell and plays an important role in the immune tolerance and tumor immune escape.In recent years,with the development of tumor immunotherapy,people start noting that Treg cells act as an important role in tumor drug resistance.Due to the important role of FOXP3 in the function of Treg cells,its relationship with tumorigenesis,immune escape,progression and prognosis has attracted more and more researchers’ attention.With the deepening of the research,people found that FOXP3 is expressed not only in Treg cells,but also in non-Treg cells and other normal tissue and even in cancer cells.By searching and reading the literature of FOXP3 related to malignant tumors,we found that FOXP3 is expressed in various cancer tissue,such as breast cancer,gastric cancer,colorectal cancer and so on,and it is generally considered as a suppressor in breast cancer.However,its research in gastric cancer is still largely unknown and the role of FOXP3 remains controversial.Therefore,the study of the expression of FOXP3 in gastric cancer and its role in the occurrence,development and prognosis of gastric cancer and clarifying its mechanism and regulation mechanism is of great significance for chemotherapy,targeted therapy and immunotherapy of gastric cancer.Aims: This study was to investigate the expression of FOXP3 in gastric cancer tissue and cell lines and to reveal that FOXP3 is involved in the invasion and migration of gastric cancer,for finding a new diagnostic marker of gastric cancer and providing a possible target for the treatment of it.Methods: Firstly,the expression of FOXP3 protein in gastric cancer tissue and cancer adjacent tissue was detected by immunohistochemistry.Secondly,the expression of FOXP3 in gastric cancer cell lines was detected by qRT-PCR,Western Blot.Thirdly,we used FOXP3 overexpression lentivirus to transfect selected cell linesand established stably FOXP3 overexpressing cells.Fourthly,transwell invasion assay and scratch assay was used to explore the effects of FOXP3 overexpression on gastric cancer cell invasion and migration.Finally,Western Blot was used to detect the expression of FOXP3 in GES-1 after Helicobacter pylori infection,in order to explore the role of FOXP3 in the development of gastric cancer.Result: FOXP3 was expressed in both gastric cancer and paracancerous tissue and the expression in adjacent tissue was significantly higher than that in gastric cancer.The expression in gastric cancer tissue was mainly located in the nucleus and cytoplasm,while the tissue in the adjacent tissues was mainly located in the cytoplasm.In addition,FOPX3 mRNA was not associated with the differentiation of gastric cancer cell lines.Compared with GES-1,the expression of FOXP3 mRNA did not decrease with the increase of the malignant degree of the cell line.The expression of FOXP3 protein in SGC-7901 was significantly higher than that in GES-1,while it was markedly lower in AGS and MGC-803.Transwell invasion assay and scratch test showed that the number of AGS cells that passed through Matrigel in the control group were significantly more than the FOXP3 overexpressed group in the invasion assay.The scratch in the control group was obviously healed and the overexpression group showed no significant change,indicating that overexpression of FOXP3 protein can inhibit the invasion and migration ability of gastric cancer cells.Helicobacter pylori co-culture with GES-1 significantly inhibited the expression of FOXP3 protein in GES-1.The expression of FOXP3 protein was decreased with the increase of helicobacter pylori concentration,indicating that FOXP3 may decrease when gastric epithelial cells was transforming into cancer cells and the result was consistent with immunohistochemistry.Conclusion: FOXP3 gene may be an anti-oncogene. |
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