Antiviral Activities Of L₅-CVN In Vitro&the Role Of N-terminal Leucine In CVN’s Binding To Glycosylated Gp41

The interaction between influenza virus HA protein and L5-CVN,L5-CVN and gp120,L5-CVNand gp41proteinwere detected in vitro.In the cellular level,the antiviral ability ofL5-CVN against A/HK/8/68influenza a strain (H3N2), A/WSN/33(H1N1) andA/Swan/Hokkaido/51/96(H5N3) was detected on MDCK cellsand the value of EC50wascalculated. Through immune fluorescent dyeing and observing using fluorescent microscope, theantiviral ability were detected by observing CPE. Inorder to observe the effect ofL5-CVN onvirus invasion, cell attachment and copy of virus in cells,virus was dealt with different way byL5-CV-N. Asa result, L5-CVN highly affiliate with HA, gp120and gp41protein in lowconcentration and the affinity rely on the concentration in certain range. L5-CVN was able toinhibit the strain A/HK/8/68(H3N2), A/WSN/33(H1N1) and A/Swan/Hokkaido/51/96(H5N3)on MDCK cells. However, for strain A/WSN/33(H1N1), the inhibitory effects are limited.L5-CVN were able to prevent the virus from infecting cells, but had no inhibitory effect onintracellular replication of the virus.Four kinds of Cyanovirin-N(CVN) mutants were constructed. The first N-terminal amino acidresidue Leu of CVN was first targeted deleted, and replaced byLys and Val. By combiningNative-PAGE with Western Blot, CVN mutants which expressed in Escherichia coli strain BL21(DE3) were isolated, purificated, and detected of the interaction with HIV-1coating membraneglycoprotein gp41.