Immunosuppressive Effects Of Demethylzeylasteral In The Rat Kidney Transplantation Model

Objectives:To investigated the immunemechanism of acute or chronic rejection in renal transplantation and selectednewimmunosuppressants, a simple and reliable kidney transplantation model in the rats, and postoperative serum creatinine (Cr) and blood urea nitrogen (BUN) of transplanted kidney.Material and methods:The procedure was performed between donor SD male rats and recipient Wistar male rats, weighing about250g(n=10). A long abdominal incision was performed from the xyphoid to the symphysis pubis. the left renal and hilum were exposed, left renal artery and vein were dissected under surgerical microscope, after blocking the aorta at the upper level of the left renal artery, the left renal was perfused with15to20ml0-4℃normal saline through catheter placed into aorta at lower level of the left renal artery. The left kidney and total ureter with bladder flap were harvested, and transfered into cold saline. After the recipient left kidney was harvested, the grafted kidney was placed orthotopically into recipient, the procedure was performed with the end-to-end anastomosis in both renal artery and vein, and bladder flap was stitched to recipient bladder, the recipient received right nephrectomy at the same time. CSA10mg/kg.d was given to every rat once in each day. the blood was taken from Canthal vein of survived rats and the kidney was harvested for biopsy after7days.Results:successful rate of operation was80%.The result showed that serum creatinine and blood urea nitrogen of transplanted kidney were stable7days after operation, there were no significant difference between normal and donor rats in serum creatinine and blood urea nitrogen(P>0.05).Conclusions:We established a simple and reliable kidney transplantation model in rats. It can be used as a model to study transplantation immunity and evaluate immunosuppressant. Objectives:To study and evaluate the immunosuppressive effect of T-96using a rat kidney transplantation model with the control of CSA and Tripterine.Material and methods:In this study, renal allografts were undertaken between SD and Wistar rats. Rats were randomly divided into7groups following kidney transplantation, and different doses of T-96,CsA and tripterine were administeredto each group:1, control group treated with normal saline (n=8);2, CsA-treated group (5mg/kg/day)(n=8);3, CsA-treated group (10mg/kg/day)(n=8);4,T-96-treated group (10mg/kg/day)(n=8);5, T-96-treated group (20mg/kg/day)(n=8);6, tripterine-treated group (0.3mg/kg/day)(n=8); and7, tripterine-treated group (0.6mg/kg/day)(n=6).Except for the blank control group, the two additional allografts rats were added to each group for biopsy at7and14day after procedure.Results:The results showed that T-96at a dosage of10and20mg/kg/day prolonged graft survival up to15.1±1.04d and21.4±2.61d respectively, and showed dose-response relationship. thedifference in survival time was insignificant between T-96and CSA group at both low and high dose(P>0.05),but tripterine prolonged shorter graft survival time compared to T-96and CSA in high dose group.(P<0.05)Conclusions:These results demonstrate the strong immunosuppressive activity of T-96and suggest a possible clinical use for T-96as an immunosuppressive agent in the fields of organ transplantation and autoimmune disorders.