Study On The Mechanism Of Tripterine Intervention In Alzh-eimer’s Disease In Rats Based On Autophagy

Objective: By observing the effect of tripterine on learning and memory ability,as well as the expression of Aβ,p-Tau,autophagy related proteins Beclin1,LC3-II expression in hippocampal neurons,the protection effects between tripterine and tripterine on Alzheimer’s disease(AD)related hippocampal neurons and the regulation of autophagy-lysosomal pathway was also compared so as to clarify the effects and mechanism of tripterine on rats AD to the support new drug development.Method: The experiments was designed to consturct a model in Alzheimer’s disease rats by3 D orientation injection of Aβ1-42 stereotactic to the bilateral hippocampa.A total of 120 SPF Wister rats aged 8weeks were selected,with aweight of about 250 g,half male and half female.20 rats were divided into the control group,the bilateral hippocampus was given the same amount of normal saline instead of the model drug.And the remaining 100 rats were randomly divided into 5 groups after unified modeling(model group AD,tripterine of high dose,middle does and low dose group,rapamycin group),each group have 20 rats,10 were used with the both side of hippocampus in HE staning,immunohistochemical determination of hippocampus Aβ,p-Tau expression,the other 10 were used for Western Blot to detectd hippocampus autophagy related proteins Beclin 1and LC3-II.Results: Compared with AD group,as the train time extended,escape latency of place test of the high does,middle dose,low dose tripterine group,was reduced(P<0.05),and the difference between high、medium dose group of tripterine was more obvious than low dosegroup.The time poportion and interval of central retention time of AD group were significantly reduced(P<0.05)while compared with control group but longer than high does,middle dose,low dose tripterine group.The expression of Beclin-1 and IL3-II was significantly lower in AD model group compared with control group.The three tripterine groups and rapamycin group express more Beclin-1 and IL3-II than AD group.The expression of Aβ and p-Tau was significantly more in AD model group compared with control group.The three tripterine groups and rapamycin group express lower Aβ、p-Tau than AD group,Based on immunohistochemical results.In the above results,the differences AD grually narrowed with the decrease of tripterine dose,and the differences in the rapamycin group were similar to those in the control group,which were all better than those in the tripterrine group.Conclusion: Tripterine could protect the brain neurons by inducing autophagy pathway as well as regulating the autophagy-lysosome pathway,learning and memory ability was improved in the rats of AD group.This research could provide a new target of tripterine in the treatment of Alzheimer’s disease as well as the research method and animal experiment theoretical basis.