Clinical Research On Method Of Invigorating Kidney To Regulate Menstrual Cycle For Isoimmune Recurrent Spontaneous Abortion

Objective:Through detecting the serum blocking antibody level、Thl cytokine and Th2cytokine of patients who had recurrent abortion induced by traditional Chinese medicine invigorating kidney to regulate menstrual cycle,to observe the clinical efficacy of the decoction and investigates its mechanism before and after treatment of invigorating kidney to regulate menstrual cycle.Method:40patients were selected to take the TCM prescription of invigorating kidney to regulate menstrual cycle, Setting3months as a course of treatment.Then to observe (1) blocking antibody-negative to be positive post treating;(2) the serum level of TNF-α、IL-6、TNF-α、IL-6in pre-and post treating;(3) the improvement of clinical symptoms in pre-and post treating;(4) blocking antibody-negative to be positive in successful pregnancy patients.Results:(1) Blocking antibody-negative to be positive:After treating,the positive rate of anti-CD3-BE was higher than pre treating,it had significant difference;Anti-CD4-BE had no significant difference in positive conversion in pre-and post treating; Anti-CD8-BE had significant difference in positive conversion in pre-and post treating;(2) Symptom scores:Comparing the before and after treatment, every symptom had statistical significance value respectively, the total score of symptoms before and after treatment were striking. TCM nourishing kidney and strengthing spleen was an effective option.(3) Blocking antibody-negative to be positive in successful pregnancy patients:pregnancy in16,15cases were successful,the rate was93.75%. The rate whose blocking antibody had all the three positive the rate was13.33%, two was53.33%,one was20.00%,all of blocking antibody-negative was13.33%.(4) the change of t helper cell type1and2cytokines:Comparing the before and after treatment, the level of TNF-a declines from183.11±47.29pg/ml to80.67±38.65pg/ml; the level of IL-6rises from3.64±1.78pg/ml to6.90±3.18pg/ml; the ratio of TNF-α/IL-6declines from63.78±37.10to15.71±13.23. Each change has significant difference.(5) For the comprehensive curative effect:Cure rate is37.50%,with significant efficiency for2.50%,with efficiency for57.50%, and the total effective rate account for97.50%.(6) Conclusion:Invigorating kidney to regulate menstrual cycle can improve the spleen and kidney weakness syndrome, and it can promote anti-CD3-BE、Anti-CD8-BE to be positive and decline the ratio of TNF-α/IL-6; Patients who were negative and had not changed to be positive can also get successful pregnancy.