The Expression And Study Of The MiR-101in The Gastric Cancer

Objective: miR-101has less effect in tumor tissues and cells expression such ascolon cancer, liver carcinoma and prostate cancer. However, its concrete effect ingastric cancer was still poorly known. This study aimed to investigate the effect of themiR-101on the gastric carlinoma and its clinical and biological significance in gastriccancer.Materials and methods：We examined miR-101expression in92paired normal andstomach tumour specimens, as well as gastric cell lines by Quantitative RT-PCRanalysis. The association between miR-101levels and clinicopathologic factors wasanalysed. Additionally, we observed the effect of miR-101overexpression onMGC-803gastric cancer cell proliferation and invasion by MTT, Transwell andWestern blot assay.Results: The expression of miR-101was strongly downregulated in gastric cancerand cell lines. The low expression of miR-101was inversely associated with theclinical stage and depth of invasion, and the expression of lymph node metastasisgroup was obviously lower than the group of non-lymph node metastasis.miR-101overexpression decreased significantly the protein levels of EZH2andDNMT3A by Western blot after48h. miR-101can significantly inhibit the growthproliferation of MGC803cells with miR-101mimics, si-EZH2or si-DNMT3A after48h. The cells number through matrix gelatin in the group of miR-101mimicssignificantly lower than the compared group, the same as the group of si-EZH2andsi-DNMT3A.Conclusions:1. miR-101expression level was downregulated in gastric cancer tissues and celllines. The low expression of miR-101has a close correlation to the depth ofinfiltration, clinical stage and lymph node metastasis.2. miR-101inhibited gastric cancer cell proliferation and invasion by targetingEZH2and DNMT3A expression.