| Objective: To analyze the mechanism and the role of miR-34a in the gastric cancercells.Methods: Cell growth was evaluated by MTT. Cell invasion potential was evaluatedby scratch test and transwell assay. The apoptosis cells were tested byAnnexinV-FITC/PI staining. Finally, western blotting was used to analyze the effectsof miR-34a on Survivin, E2F3, Bcl-2, CD44protein expression.Results: The MTT assay demonstrated that after overexpressing miR-34a in gastriccancer cells, cellular viability was significantly reduced (p<0.05). The Transwellinvasion chamber assay illustrated that after increasing the expression of miR-34a, thenumber of cells passing through the Transwell chamber were significantly reduced(p<0.01). Flow cytometry analysis determined that upon miR-34a overexpression, theapoptosis cells increased significantly. Based on western blotting, compared with thecontrol group, Survivin, Bcl-2, CD44protein expression levels were significantlydecreased in the gastric cancer cells transfected with the miR-34a mimic for48h.Conclusions:1. miR-34a can inhibit gastric cancer cell proliferation, invasion and inducedapoptosis.2. miR-34a can decrease Survivin, Bcl-2and CD44protein expression in MGC-803cells. |
