| Objective:1.To observe the popliteal fossa lymph nodes’s structure, cell morphology andthe neogenesis of vessels and lymphangions in SD rats,which were under the manualintervention factors,such as ligature popliteal fossa lymph nodes’s artery, vein andtheir afferent lymphatic, efferent lymphatic.2.To explore the mesenteric lymphatic obstruction of the New Zealand’s whiterabbit on the impact of lymph node.3.To lay a good foundation for the subsequent tumor metastasis lymph nodeligation models.Methods:The first part: the chance of the lymph nodes after ligation the SD rats.1.Divided into groups. The experimental groups were48SD rats,which weredivided into three groups equally and randomly,the processing factors of three groupswere vascular ligation, lymphatic ligation and both ligation;the control group was therest4SD rats.2.Established ligation models.The experimental groups rats were all injectedmethylthioninium chloride0.2ml into the rat paw after complexing iodinedisinfection for tagging lymph nodes and lymphatic vessels. The first group ligaturedpopliteal fossa lymph node’s artery and vein in rats;the second group ligaturedpopliteal fossa lymph node’s input and output lymphatic in rats;the third groupligatured all vessels and lymphatics. 3.Fixed, embeded, sectioned and stained. In the above three groups of rats, fourwere put to death from each group respectively on the day3, day7,day14and day21;the control group rats were put to death in day3.All groups of rats were excisedthe popliteal fossa lymph nodes after death, measured the length,then stained by HEstaining, CD31and D2-40immunohistochemical staining for diagnosis.4.Analyzed statistics.The SPSS19.0statistical software was uesd.Examined thenormality and homogeneity of variance analysis. Measurement data statisticalanalysis used mean±standard deviation (x±s), significant standard was p=0.05(bilateral), lymph node degree of atrophy and microvascular density (MVD) in lymphnode tissue and the lymphatic vessel density (MLD) statistics were used in analysis ofvariance.The second part:the mesenteric lymphatic obstruction effect on lymph node lymphsinus-venous anastomosis.1.Divided into groups. According to the length of time,the12New Zealand’srabbits were divided into4groups at random-1,3,7,14days.2.Showing the mesenteric lymph nodes:injected the meilan to show themesenteric lymph nodes from rabbit mesenteric root, the aizen mesenteric lymphnodes and lymphatic vessels will be visible.3. To setting-up the New Zealand’s white rabbit mesenteric lymphaticobstruction model: injecting the layered filtering Chinese ink from the afferentlymphatic duct of the mesenteric lymph nodes after the efferent lymphatic vesselwere ligated4. Looking for the carbon composition after centrifugal of the portal vein bloodat the predetermined time point. Results:The part one1.Lymph node macro changes：1.1. lymph nodes’s volume contraction after Ligation, the degree of atrophyhad positively correlation with the time of ligation,the degree of lymph node atrophwas that,21days group>14days group>7days group>3days group> the controlgroup, p <0.05, there were statistically significant differences. In all ligation groupthe volume reduced the most, vascular ligation group followed, lymphatic ligationgroup reduced the least. The differences among these groups had no statisticalsignificance (p>0.05).1.2. there were no obviously blood vessel and lymphatic neogenesis aroundthe lymph nodes after ligation.2. The structure and cell composition of lymph node changed significantly.2.1. Vascular ligation groups:21days after ligation, lymph node highendothelial cells became flater, high endothelial venules almost degradated todisappear.2.2. Lymphatic ligation groups: fewer sinus tissue cells (macrophages began toreduce in the7th day after ligation and gratully to disappear; and the lymphocytesdecreased obviously in lymph node until the21th day after ligation).2.3. All the ligation groups:7days after ligation, lymph node lymph folliclesbegan to shrink; Germinal center narrowed, tended to disappear; Macrophages,lymphocytes and high endothelial cells with the extension of ligation time showed atrend of decline.3.MVD changes: the SD rats’s popliteal fossa lymph nodes after vascularligation, lymph node MVD in the short term (3to7days) increased (p＜0.05),butthe longer duration of ligation (17-21days), MVD showed a trend of decline in atrophy. And ligation lymph nodes in the input and output lymphatic alone had nosignificant effect on the variation of MVD (p＞0.05).4.MLVD changes: ligatured SD rats’s popliteal fossa lymph nodes in the inputand output lymphatic vessels, lymph node MLVD reduced(p＜0.05), there was nosignificant difference in MLVD by the time of ligation. And ligatured lymph vesselsseparately that couldn’t influence lymph node MLVD obviosely (p＞0.05).The part two1rabbit was founded the carbon composition in the portal vein blood from the1day-group, the rest were not found the components; In the3days-group and the7days-group,2rabbits found the carbon composition at each groups;The all3rabbitsfound the carbon composition in the14days-group. the portal venous blood whichContains carbon grain became turbidity after centrifugal, and the carbon grain can bediscovered in the Blood smear.Conclusion:1.Ligatured either popliteal fossa lymph vessels or lymphatic vessels in SD rats，or both，that resulted in lymph gland atrophy and cell components reduced.As theextension of the time of ligation,the lymph node lost its normal structure.2.Ligation of the lymph node can restrain the level of the MVD and the MLVD.3.Completely ligatured either lymph vessels or lymphatic vessels，or both，thatmight have the lymph node cleaning effect. |
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