| Objective: Explore the expression of hypoxia-inducible factor-1α(HIF-1α) in rectal cancer。And research the relations that hypoxia-inducible factor-1α(HIF-1α) has with micro lymphaticvessel density (MLVD) and colorectal cancer, lymph node metastasis。In order to further understand whether the HIF-1α, MLVD the mechanism of lymphatic metastasis in rectal cancer play a role and look forward to exploring the molecular level from the rectal cancer surgery to adapt to sign。Method: Select the First Affiliated Hospital of Anhui Medical University in 2000 -2003 years between surgery and pathologically confirmed in 40 cases of low rectal cancer specimens as the experimental group, all patients were not receiving chemotherapy or radiotherapy before surgery, and a complete follow-up data。The other 40 cases taking the corresponding adjacent normal tissues as control group。Were measured by immunohistochemical staining tumor tissue and adjacent normal tissue HIF-1α,MLVD expression levels; the same time, application of CK-20 immunohistochemical staining of the experimental group routine HE staining negative lymph node micrometastases situation。Analysis of these indicators and rectal cancer size, gross type, histological type, preoperative distant metastasis, histological grade, depth of invasion, clinical staging, prognosis, surgery and postoperative recurrence anus relationsResult: 1, Rectal tissue HIF-1αpositive rate for the77385.72, normal adjacent tissue HIF-1αpositive rate for the33091.96, rectal tissue HIF-1αexpression in the average water rectal was significantly higher than in normal adjacent tissues (P <0.05); rectal tissue MLVD 7.26, (maximum8.8, minimum6), normal tissue adjacent to MLVD 0.26 ( maximum0.80, minimum0.00), rectal cancer tissues MLVD expression were significantly higher than normal adjacent tissues (P <0.05 ); Lymph node micrometastases-positive rectal cancer, cancer adjacent tissue HIF-1α, MLVD expression level was significantly higher than those with negative lymph node micrometastases in rectal cancer organizations and cancer tissues, the difference is statistically significant (P <0.05)。2,Using tests to detect colorectal cancer tissue HIF-1α, MLVD expression levels with tumor size, depth of invasion, gross type, histological type, clinical stage, lymph node micro-metastasis, preoperative distant metastasis, differentiation, etc。the relationship between clinicopathological characteristics and found that HIF-1α, MLVD expression and characteristics of no significant correlation (P> 0.05), but with the characteristics of significant correlation (P <0.05)。3,Using tests to detect lymph node micrometastases in lymph node micrometastasis-positive group and negative group in HIF-1α, MLVD the expression level of statistically significant differences (P <0.05)。4,Using Cox regression univariate analysis of clinicopathologic factors and cancer of HIF-1α, MLVD level and the relationship between the prognosis and found that characteristics of the above indicators into the Cox regression proportional hazard model analysis, we found HIF-1α, MLVD degree of differentiation, lymph node micrometastasis is an independent factor affecting the prognosis of rectal cancer (P <0.05)。5,Using analysis of variance and chi-square test analysis of rectal cancer recurrence and HIF-1α, MLVD and lymph node micro-metastasis showed that HIF-1α, MLVD high expression and lymph node micro-metastasis-positive cases more easily after recurrence.Conclusion : 1, HIF-1αand MLVD play an important role in the development in the incidence of colorectal cancer, suggesting HIF-1αmay be plays a catalytic role by VEGF-C-VEGFR3 channels or other means of pairs of colorectal cancer in the formation of MLVD。2, There is micrometastasis in lymph node metastasis of colorectal cancer, HIF-1αand MLVD play in some important catalytic role in micrometastasis。3, Through the patients were followed up, HIF-1α, MLVD expressed a strong chance of cases of local recurrence in patients with poor prognosis, shorter survival time。Preoperative detection of HIF-1α, MLVD rectal cancer may provide the molecular basis。4,HIF-1α, MLVD high expression in some way is the promotion of lymph node micrometastases in colorectal cancer cases, suggesting HIF-1α, MLVD likely targeted therapy can be used as the molecular basis of cancer。... |
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