Experimental Study On The Prevention Of Renal Calcium Oxalate Calculus In Rats By Flavonoids From Mosla

ObjectiveUsing SD male calcium oxalate stone rats which induced by glycoland ammonium chloride as models,interfering by Mosla flavonoidsextract to discuss its influence and mechanism in SD rats of calciumoxalate stone,which provides material and ideas for prevention of calciumoxalate stone.Methods1.The rat renal calcium oxalate calculi model were established by gavage1%ethylene glycol and1%ammonia chloride.2. Forty of health SD male rats randomly were divided into fivegroups(N=8each). group A (normal control group); group B(group stoneformation); group C(small-dose treatment group); group D(median-dosetreatment group); group E(high-dose treatment group)3. Each treatment group was given100,200,400mg/kg Mosla flavonoidsextract orally for two weeks. Use potassium chromate oxidation catalyticspectrophotometric determination to determine oxalic acid redconcentration and content of rat urine. Use automatic biochemicalanalyzer serum and urine urea nitrogen (BUN), Cr, and Ca2+，P3+，K+，Na+content. Use atomic absorption spectrometry to test renal tissue Ca2+, Mg2+level.View distribution of calcium oxalate crystals and tubulardilatation in the renal tubules under the digital optical microscope.ResultsDifferent doses of flavonoids extracted Mosla intervention group renaltissue Ca2+level,24h urinary Ca2+excretion, calcium oxalate crystaldeposition and tubular dilatation of the renal tissue were significantlylower than calcium oxalate stones in the model group（P<0.05）. Therewas no significant difference between the intervention chestnut extractflavonoids limonene rats urinary oxalate and oxalic acid content and theconcentration of calcium oxalate stones24h model group involvement（P>0.05）.Conclusions1.The method of1％ethylene glycol plus l％ammonia chloride for twoweeks could induce the formation of renal calcium oxalate calculusmode;2. Mosla flavonoids extract could beeffective in controlling the formationof renal calcium oxalate crystallization for SD male rats;3. Calcium metabolism in rats with experimental intervention may be oneof the effectivewaysfor Mosla flavonoid extract inhibits renal calciumoxalate crystal formation in rats.