Inhibitory Effects And Related Mechanisms Of EGCG On Renal Calcium Oxalate Formation In Rats

Objective:To explore the inhibitory effects and related mechanism of EGCG on renal calcium oxalate calculus.Methods:Rat model of renal calculus was induced by oral administration of ethylene glycol and ammonium chloride which received EGCG treatment. Levels of blood creatinine (Cr), blood calcium and blood urea nitrogen (BUN) were detected; levels of calcium and oxalic acid (Ox) were assessed; histological evaluation of pathological changes of renal calculus were assessed; expression levels of OPN were evaluated by real-time PCR and Western Blotting.Results:Compared with normal rats, the levels of Cr, BUN,urine calcium, urine Ox and renal OPN expression increased significantly (P<0.05),24-hour urine volume decreased significantly (P<0.05); compared with rats with renal calculus, the levels of Cr, BUN, urine calcium and urine Ox decreased significantly (P<0.05),24-hour urine volume increased significantly (P<0.05) after EGCG treatment in a concentration-dependent manner. Additionally, compared EGCG inhibits formation of calcium oxalate calculus in experimental animal model and exert dramatic renal protective effect, which was related with attenuation of renal OPN expression ner (P<0.05).Conclusion:EGCG inhibits formation of calcium oxalate calculus in experimental animal model and exert dramatic renal protective effect, which was related with attenuation of renal OPN expression.