| Objective: To investigate the role of vitamin K-dependent carboxylase (VKDC) in onset of calcium oxalate calculus through the detection of all exons in patients'nephridial tissue. Meanwhile, we want to approach the application of DHPLC in detection of mutation.Method: By using PCR and denatured high pressure liquid chromatography(DHPLC), we detected mutation in all exons locating in encoding region of VKDC gene of 70 controls and 44 calcium oxalate calculi patients, and confirmed it by sequencing.Result: No genetic mutation was observed of VKDC domain in patient with calcium oxalate calculus compared to those controls.Conclusion: The formation of calcium oxalate calculus is seemed not related to the genetic mutation of domain of VKDC. And DHPLC is a rapid,high-flux,automatic and economic method of detection of mutation. |
