The ERG Features In Diabetic Rats By Using Ozone Treatment

Objective:To investigate the ERG features in diabetic rats by using ozone treatment.Methods:After experimental diabetic models were induced successfully with streptozotoein,55diabetic rats were averagely divided into3groups at random, which including ozone treatment group (group A), pure oxygen treatment group (group B) and diabetic control group (group C). Furthermore,18eyes of healthy rats were chosen as normal control group (group N). After6weeks of adapted feeding, the group A were given the mixed gas by rectal perfusion every3days, and at the same time, the group B was given pure oxygen, and the other two groups with no intervention. Period of treatment is one mouth. The ERG features including amplitudes and latencies of a-wave and b-wave were analyzed by one-way ANOVA and two more groups of mean comparison.Results:Analysising the differences of amplitudes and latencies of a-wave and b-wave in dark adaption and in bright adaption by one-way ANOVA. Amplitudes of a-wave and b-wave in dark adaption:group B (a-wave:10.44±0.97;b-wave:32.91±2.61), group C (a-wave:10.72±1.06; b-wave:33.43±2.76) are both lower than group N (a-wave:39.61±1.30; b-wave:99.45±2.77), group A (a-wave:18.68±0.92; b-wave:46.86±2.53) decreased unconspicuously, the differences were of statistical significance (P<0.05); latencies of b-wave in dark adaption:group A (42.91±4.72) was slightly shorter than group C (54.60±2.27) and group B (53.40±4.97), but there was no statistically significant differences(P>0.05); latencies of a-wave in dark adaption:group B (30.10±2.42), group C (30.30±2.58) were extended than group N (13.44±2.55), group B (19.81±2.71) extended unconspicuously, the differences were of statistical significance. In bright adaption, there was no statistically significant difference in the amplitudes of a-wave(F=0.28, P>0.05); amplitude of b-wave:group B(15.01±0.87), group C(14.18±0.79) were lower than group N (25.41±1.25), group A (21.82±0.78) decreased unconspicuously, the difference was of statistical significance (P<0.05); latencies of a-wave:group A (15.72±1.90), group B (15.40±2.01) and group C (15.30±1.63) are all slightly extended, but there was no statistically significant difference for any two groups (P>0.05); latencies of b-wave:group B (65.20±1.54), group C (65.30±1.94) are obviously extended than group N (43.00±2.82), group A (51.63±2.87) extended slightly (51.63±2.87), the difference was of statistical significance (P<0.05).Conclusion:Functional changes are appeared in the early stages in diabetic rats, rectal gas injection of ozone treatment may show protective effects on the nerve function in diabetic retina.