Effects Of Hedyotis Diffusa Wild On Human Retinal Capillary Endothelial Cells’ Proliferation And Expression Of VEGF

ObjectiveThe intention was to probe the effect of human retinal vascular endothelial cellproliferation and its VEGF expression on hedyotis diffusa injection and its angiogene-sis inhibition mechanism in high glucose environment,and to provide a new treatme-nt for Diabetic retinopathy.MethodsF Ⅷ r-Ab was identified HRCECs which were cultured in vitro.The culturedHRCECs were dealed with different concentration of hedyotis diffusa injection,theinfluence of cell proliferation was surveyed by CCK-8.The VEGF expression ofHRCECs was identified by using ICC(Immunocytochemistry) test and ELISA(Enzy-melinked immunosorbent assay).Results1.ICC assay indicated that in HRCECs the expression of F Ⅷ r-Ag was positive.2.CCK-8assay indicated that with6.25ml/L、12.5ml/L、25ml/L、50ml/L HDI treatmentunder high glucose,it inhabited the ability of HRCECs proliferation dependanting ontime and dose,except6.25ml/L HDI group.The inhibitory rates were3.58±1.06%、8.92±3.32%、15.68±3.18%、33.17±2.49%after effected by HDI on24hours;they are4.17±1.53%、14.09±2.98%、31.40±3.71%、45.95±2.63%after48hours and4.53±1.09%、24.18±6.53%、47.62±2.96%、63.14±3.37%after72hours;the differencebetween HDI treatment groups and high glucose control group was significant(P<0.05),as soon as the difference between the high glucose and the low glucosecontrol group(P<0.05).3. ICC test and ELISA assay both showed that with12.5ml/L、25ml/L、50ml/L HDI treatment, after72hours,the difference of the picture with thegray value and OD value was statistics significant (P<0.05), the same as thedifference between the high glucose and the low glucose control group (P<0.05). ConclusionHDW inhibited the proliferation HRCECs dependenting on dose and time;it caninhibit angiogenesis by decreasing the VEGF expression. It has important applicationsin the treatment of retinal neovascularization in DR.