| 【Objective】:To study the expression and change of thioredoxin1and thioredoxinreductase in aged rats of chronic obstructive pulmonary disease, and furtherinvestigate the role of thioredoxin system in anti-oxidative in senile patients withchronic obstructive pulmonary disease.【Methods】Male SpragueDawleyratswererandomlydividedintotwogroups:adult(6months old) group and aged (18months old) group. Then each group were dividedinto normal control group、COPD model group、PX-12treated group、neurotropintreated group and every group had10male SD rats. In every group, the lung functionmeasurements were carried out, the pathological changes of lung were observed, andthe expression levels of ROS were detected. mRNAs and proteins expression ofTRX1、TRXR were measured. Proteins expression of TRX1、TRXR、GSH1、γ-GCSin bronchoalveolar lavage fluid (BLAF) and plasma were also detected.【Results】The results of rats pulmonary function showed:peak expiratory flow(PEF)、forced expiratory volume in first0.3second (FEVO.3) and percentage of forcedexpiratory volume in first0.3second to forced vital capacity/(FEVO.3/FVC%) wereall decreased in aged group compared with adult group but had no statisticalsignificance (P＞0.05). Except for lung pathological in adult control and agedcontrol had no obvious changes,pathological of rats lung tissue in aged groupmarkedly behaved worse compared with adult group. In aged group, ROS levelswas markedly raised compared to adult controls.(P＜0.01) Compared with adultgroup,mRNAs expression of TRX1and TRXR were all decreased in aged group.(aged conrol group compared with adult control group,P>0.05; except for theexpression of TRX1in aged PX-12treated group compared with adult PX-12treated group and aged neurotropin-treated group compared with adult neurotropin-treatedgroup,P<0.01, the others showed aged group compared with adult group,P<0.05);And compared with adult group,proteins expression of TRX1、TRXR in aged groupwere all markedly decreased.(aged control group compared with adult control group,P>0.05; except for the expression of GSH1in aged neurotropin-treated groupcompared with adult neurotropin-treated group, P<0.01;the others showed that agedgroup compared adult group, P<0.05). In immunohistochemical showed that theposition of TRX1、TRXR proteins expression were mainly located in rats alveolarwalls、bronchial epithelial cells in nearly every groups. Whereever, the proteinsexpression in aged groups were decreased compared with adult groups. Inenzyme-linked immunosorbnent assay (ELISA) showed that the proteins expressionof TRX1，TRXR，GSH1，γ-GCS in plasma were obviously decreased in aged groupscompared with adult groups.(aged control groups compared with adult control group,P>0.05; except for the expression of TRX1in aged PX-12treated group comparedwith adult PX-12treated group,P <0.01;the others showed aged group compared withadult group, P<0.05)；In enzyme-linked immunosorbnent assay (ELISA) showed thatthe proteins expression of TRX1，TRXR，GSH1，γ-GCS in bronchoalveolar lavagefluid (BLAF) were markedly decreased in aged groups compared with adult groups.(aged control group compared with adult control group, P>0.05; except for theexpression of GSH1in aged COPD group compared with adult COPD group, theexpression of TRX1、TRXR and GSH1in aged PX-12treated group compared withadult PX-12treated group,the expression of GSH1in aged neuorotropin-treated groupcompared with adult neurotropin-treated group showed P<0.01; the others showedthe aged group compared with adult group, P<0.05).【Conclusions】TRX1and its reductase can remove the excessive reactive oxygenspecies and play antioxidant effect in rat COPD disease.TRX1and its reductasedecreased in the aged rat COPD disease, making its antioxidant capacity decline. |
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