Association Between RYR3Genetic Polymorphism And Atherosclerosis Cerebral Ischemia

Background and objective:Atherosclerosis cerebral infarction (ACI) is one of the greatest damaging disease of the world, its high incidence, high morbidity, high mortality rates have a serious impact on human life and quality of life. There are many causes of ACI, atherosclerosis (AS) is an important cause and pathology of ACI. In recent years, studies abroad have shown that RYR3gene through regulating the Ca2+channel to participate ACI, but there are no studies in China. The purpose of this study is to analyze the relationship between the polymorphisms of three single nucleotides(SNPs) in RYR3gene and ACI patients from Hunan Han population.Methods:We performed the case-control genetic association study, using polymerase chain reaction(PCR) and matrix-assisted laser desorption ionization time of flight mass spectrometry to analyze the distribution and interaction of rs877087. rs2229116and rs2132207in RYR3gene genotype, allele and haplotype in China’s Hunan Han population comprising of228ACI patients and233healthy controls.Results:1. The frequencies of CC, CT, TT genotypes of RYR3gene in rs877087locus were0%.9.7%,90.3%respectively in patients with ACI, and0%、10.3%、89.7%respectively in control subjects. None significantly difference in the distribution of genotypes between the ACI group and the controls (P>0.05). The frequencies of C, T allele were 4.8%.95.2%respectively in patients with ACI, and5.2%.94.8%respectively in control subjects. None significantly difference in the distribution of allele analysis between the ACI group and the controls too (P>0.05).2. The frequencies of AA、AG、GG genotypes of RYR3gene in rs2229116locus were71.5%、27.2%、1.3%respectively in patients with ACI, and71.2%、25.3%、3.4%respectively in control subjects. None significantly difference in the distribution of genotypes between the ACI group and the controls (P>0.05). The frequencies of A, G allele were85.1%、14.9%respectively in patients with ACI, and83.9%、16.1%respectively in control subjects. None significantly difference in the distribution of allele analysis between the ACI group and the controls too (P>0.05).3. The frequencies of CC、 TT、CT genotypes of RYR3gene in rs2132207locus were40.9%、10.7%、48.4%respectively in patients with ACI, and44.4%.13.8%.41.8%respectively in control subjects. None significantly difference in the distribution of genotypes between the ACI group and the controls (P>0.05). The frequencies of T, C allele were4.9%.65.1%respectively in patients with ACI, and34.7%.65.3%respectively in control subjects. None significantly difference in the distribution of allele analysis between the ACI group and the controls too (P>0.05).4. The frequencies of AA+AG. GG genotypes of RYR3gene in rs2229116locus were98.7%.1.3%respectively in patients with ACI, and96.6%.3.4%respectively in control subjects.. The frequencies of AA. AG+GG genotypes of RYR3gene in rs2229116locus were71.5%. 28.5%respectively in patients with ACI, and71.2%、28.8%respectively in control subjects. None significantly difference between the ACI group and the controls too (P>0.05). The frequencies of CC. TT+CT genotypes of RYR3gene in rs2132207locus were40.9%、59.1%respectively in patients with ACI, and44.4%、55.6%respectively in control subjects.. The frequencies of TT. CC+CT genotypes of RYR3gene in rs2132207locus were10.7%、89.3%respectively in patients with ACI, and13.8%、86.2%respectively in control subjects. None significantly difference between the ACI group and the controls too (P>0.05).Conclusions:1. There are no obvious association between the rs877087、 rs2229116and rs2132207of RYR3gene polymorphism and ACI attacking in Hunan Han population of China.2. The homozygous of RYR3gene are not associated with the attacking of ACI in Hunan Han population of China.