Studies On The Chemical Constituents Of Acetyl Acetate Extracted Fraction From Veratrum Maaekii Regel

Veratrum.maackii Regel is one of species of the genus Veratrum (Liliaceae), according to the "flora Chinese " records:Veratrum maackii Regel is mainly distributed in the northeast, Shandong, Inner Mongolia etc. Veratrum is a perennial herb, there are about40species, mainly distributed in Europe, Asia and North America, there are thirteen species and one varieties in china, mainly distributed in North China, northeast, southwest, East China, South China.. The roots and rhizomes of several Veratrum species, including Veratrum. nigrum L.; Veratrum. japonicum(Baker)Loes. f.; Veratrum. dahuricum(Turcz.)Loes. f.; Veratrum. maackii Regel; Veratrum. Schindleri Loes. f.; Veratrum. taliense Loes. f. have been used to treat stroke, headache, epilepsy, dysentery, jaundice, scabies and other diseases as Traditional Chinese medicine.Nowadays, the hot research on new drug is to extract the chemical constituents with pharmacological activity from natural products, Chinese scholars make a lot of pharmacological studies on the chemical constituents of Veratrum plants, they have found that the steroidal alkaloids of Veratrum plants have the effects of lower blood pressure, antithrombosis, antitumor, enhance myocardial contractility. Although many researchers have been did a lot studies on chemical constituents, pharmacological and toxicological of Veratrum plants, the researches are not thoroughly and not wide enough on the whole. Veratrum plants is a kind of poisonous Traditional Chinese Medicine, to play its pharmacological effects as well as certain toxic effects, Modern research proves that,Veratrum alkaloids are the effective ingredients and toxic constituents of Veratrum plants, Therefore due to the toxicity of Veratrum plants has greatly limits the application of Veratrum plants in the clinical and the development of new drugs, our previous study found that, Veratrum alkaloids can cause DNA damage of brain cells in mice, So on the existing basis, it is necessary to make a futher and more system study on the active constituents of Veratrum plants continuing to extract monomer compounds from Veratrum plants, finding out the chemical composition of pharmacological activity with highly efficient and low toxicity, to further clarify the chemical composition of Veratrum plants, accumulation data for futher study of natural medicine chemistry and laid the material foundation for further study on pharmacology research, toxicological studies and Structure toxicity relationships studies of Veratrum plants.Having reviewed the extensive literature found that, so far,more studies on the chemical constituents of Veratrum. nigrum L; Veratrum. japonicum(Baker)Loes. f.; Veratrum. stenophyllum Diels; Veratrum. dahuricum(Turcz.)Loes. f.; Veratrum. Schindleri Loes. f.; Veratrum. nigrum L. var.ussuriense Nakai; Veratrum. grandiflorum (Maxim.) Loes. f.; Veratrum. taliense Loes.f., while the studies on the chemical constituents of Veratrum. maackii Regel relatively weak. In view of this, my thesis did a lot of research on the alkaloid constituents of Veratrum maackii Regel ethyl acetate parts, so as to extract the new steroidal alkaloids, provide monomer compounds for the study of toxicity and the toxic mechanism of Veratrum plants, and make a basis of chemical composition for the develop and utilize of Veratrum plants.The chemical constituents of ethyl acetate fraction of Veratrum maackii Regel were studied in this article, First, herbs refluxing extraction by95%ethanol, then,ultrasonic extraction with chloroform and then extraction by chloroform and ethyl acetate, The ethyl acetate fraction is obtained.By using column chromatography with silica gel, macroporous adsorption resin (HPD100) and dextran gel column (Sephadex LH-20) and preparative TLC, preparative HPLC chromatographic technique to separate compounds, At the same time with5%sulfuric acid ethanol reagent and bismuth potassium iodide reagent, Mainly to extract the alkaloid constituents of Veratrum maackii Regel from ethyl acetate extract.By using nuclear magnetic resonance spectroscopy and mass spectrometry (1H-NMR,13C-NMR,’H-’HCOSY, HMBC, HMQC, NOESY, ESI-MS) to identify the structure of compounds which are isolated, eleven compounds are isolated and all of them are alkaloids, Among them,identification the structure of nine compounds,they are3-O-β-D-glucoside-23-methoxylcyclopamine(L1); verussurine(L2); verabenzoamine (L3); germerine(L4); verazine(L5); isoverazine(L6); veramitaline(L7); Tomatillidine(L8); jervine(L9). L1is a new compound. L8is isolated from the Veratrum plants for the first time. L2, L3, L4, L7, L9are isolated from Veratrum. maackii Regel for the first time.