ObjectiveTo investigate the expression of glucose-regulated protein78(GRP78) in human laryngeal squamous cell carcinoma, adjacent to carcinoma and normal laryngeal squamous epithelial tissue.In addition, the relationship between GRP78expression and clinical parameters of patients, such as gender, tumor differentiation grade stage, and lymphatic metastasis was analyzed. and to evaluate its role in the development progress of human laryngeal squamous cell carcinoma. Materials and methods1.90cases LSCC tissue with paraffin embedding (surgically resected specimens) and adjacent to carcinoma and normal mucosa tissues with complete resection and clear pathological diagnosis were selected which came from the department of otolaryngology Head and Neck Surgery of the first affiliated hospital of Zhengzhou University from January2010to January2011. All patients were not carried out prior to radiotherapy, chemotherapy and other anti-tumor treatment.2. Using immunohistochemical streptomycin avidin peroxidase-link (SP) method, the expression of GRP78in LSCC was observed and its relationship with the occurrence and development of LSCC was analyzed. The statistics data were processed by SPSS17.0.The difference GRP78expression level of the normal laryngeal squamous epithelial tissue, matchedpair pericarcinomatous tissue and LSCC were contrast using the Kruscal-wallis. The difference GRP78expression level of the different LSCC patients with different clinicopathological parameter were contrast using the wilcoxon and Kruscal-wallis rank rest. The inspection level a was0.05. Bonferroni test was used when the pair comparison after Kruscal-wallis rank rest.The inspection level a was0.05/3. Results1. The GRP78expression level of the normal laryngeal squamous epithelial tissue, matchedpair pericarcinomatous tissue and LSCC were different (H=143.476, P<0.001). The equally GRP78expression level of LSCC and the matchedpair pericarcinomatous tissue were higher than that of the normal laryngeal squamous epithelial tissue. The positive expression rates of GRP78in LSCC, matching tissue adjacent to carcinoma and normal laryngeal mucosa tissue were separately86.67%(78/90),32.22%(29/90) and8.89%(8/90).2. The difference of GRP78expression level in LSCC in different gender people was no statistical significance(Z=0.634,P=0.526). The difference of GRP78expression level in the degree of tumor differentiation was statistical significant (H=56.995, P<0.001). The difference of GRP78expression level in the clinical stage was statistical significant(Z=2.993, P=0.003). The difference of GRP78expression level in the lymph node metastasis or not was statistical significant (Z=4.137, P<0.001). The GRP78expression level was higher in the lower the degree of tumor differentiation, the later clinical stage and Lymph node metastasis in human LSCC. Conclusion1. GRP78is closely related to the occurrence, development, transfer of LSCC, and may play an important role in the process of LSCC. GRP78can be used as a valuable molecular markers in indicating of LSCC development and measuring its malignant degree.2. The high expression rate of GRP78in LSCC may prompt a new way of thinking in the gene therapys to LSCC by inhibiting the expression of GRP78because that may inhibited the growth of cancer cells. |