Research On The Method Of Quality Control Of Yao Medicine Ardisia Crispa

Ardisia crispa is the whole dry plant of Ardisia crispa (Thunb.) A. DC.(Myrsinaceae),has been used as common folk medicinal material in Guangxi Yao for a long time.But it has not been recorded in Chinese Pharmacopeia neither in local medicinal standard.And there is also no report for quality control method of it.Quality contro for medicine is the basement on security and validity of madicine. Therefore, it is imperative to carry out research on quality control method of Ardisia crispa.This present study was designed to do systematical research for quality control method of Ardisia crispa.,using12batches medicinal material of Ardisia crispa which were collectting from different area in Guangxi as the samples, providing scientific basis for the quality standard formulation of Ardisia crispa.The main results obtained are as follows:1, Determined the base source of Ardisia crispa is the whole drying plant of Ardisia crispa (Thunb.) A. DC.of Myrsinaceae.2, Determined the method of medicinal traits identification was:the skinregion of root is thick, easy to be broken, from the cross section the wood and the skin easy to be separated; Rootstock was intamescentia slightly; stems was brown or green with thin vertical lines; Leaf margin revolute with margin glandular dots.3, The method of microscopic identification was established, key points were:the cortex account for most space at ransverse section of root, the secretory cavity was saw in cortex and pith of stem and leaf midrib and powder, the stomatals was infinitive, the number of accessory cell was2-4.4, The detection result of12batches of sample:moisture was9.3-11.8%, average10.8%; total ash was1.7-3.9%, average2.5%; acid-insoluble ash was0.1-1.3%, average0.6%; alcohol soluble extract is8.5-24.9%,average17.8%.5, The thin layer chromatography(TLC) method was established to identity bergenin and ardisiacrispin A in Ardisia crispa. The TLC system:(1) using bergenin as the reference substance,chloroform-butyl acetate-methanol-formic acid (5:2:2:1) as the spread solvent,The fresh mixture of1%potassium ferricyanide ferric chloride solution-2%ethanol solution (1:1)as the chromogenic agent;(2) useing ardisiacrispin A. as the reference substance,n-butyl alcohol-water-acetic acid (3:1:1)as the spread solvent,10%sulfuric acid ethanol solution as the chromogenic agent.The spots in the TLC were clear and distinguishable.6, The high performance liquid chromatography-end stage liver disease(HPLC-ELSD) method was established to determine the content of bergenin and ardisiacrispinA in Ardisia crispa,The HPLC system consisted of Kromasi C18100A column(250mm×4.6mm,5μm), column temperature:35℃, acetonitrile:tmethanol-water.gradient elution,flow rate of0.8mL/min;The ELSD system consisted of gas flow rate:2.5L/min, drift tube temperature:105℃;the reference substance injection volume:5μL,20μL,the sample size:5-20μL.The calibration curves of bergenin and ardisiacrispin A were ligear in range of0.1608-2.01μg (r=0.9995) and0.5058-6.323μg (r=0.9997),respectively.The average recovery of bergenin and ardisiacrispin A were103.2%(RSD=1.7%) and98.0%(RSD=1.0%),respectively. On the basis of establishing HPLC-ELSD method,the content of bergenin and ardisiacrispin A in12batches of samples were count.,the content of bergenin is0.04%-0.04%, average0.14%; the content of ardisiacrispin A was0.10%-0.10%, average0.28%. Account of the locality of thel sample were varied, it was proposed that the content limit was:the content of bergenin shall not be less than0.030%, the content of ardisiacrispin A shall not be less than0.090%.