| Objective: Through the experimental studying on quality standards of Ardisia gigantifolia Stapf and Ardisia punctata Lindl from ethnic herbs in Guangxi province,tentatively setted the quality standards of Ardisia gigantifolia Stapf and Ardisia punctata Lindl,and HPLC fingerprint of method.To provide reliably basis for the quality control of the medicines.Methods:(1)According to the appendix in the Chinese Pharmacopoeia of 2015 common rule,determined the the moisture,ash content and extract in Ardisia gigantifolia Stapf and Ardisia punctata Lindl.(2)The heavy metals of contents were determined by ICP-MS with Microwave Digestion of Cu,As,Hg,Cd,Pb in the Ardisia gigantifolia Stapf and Ardisia punctata Lindl.(3)The Gallic acid was reference sample,to refer the method of Pharmacopoeia to be identified the medicinal material by TLC.(4)The contents of Gallic acid were determined by HPLC of different origin and acquisition time in the Ardisia gigantifolia Stapf and Ardisia punctata Lindl from Guangxi.(5)The common patterns were obtained by the similarity software for analysis of different origin herbs.Results:(1)The moisture of content for Ardisia gigantifolia Stapf was from 5.65% to 12.77%;the total ash of content was from 1.54% to 2.63%;the acid insoluble ash of content was from 0.07% to 0.62%;the extract of content was from 10.14% to 17.36%.The water of content for Ardisia punctata Lindl was from 10.69% to 12.50%;the total ash of content was from 4.07% to4.85%;the acid insoluble ash of content was 0.32% to 1.05%;the extract ofcontent was from 12.03% to 17.94%.(2)The heavy metals of contents for Cu,As,Hg,Pb in Ardisia gigantifolia Stapf were in line with standard requirements.the contents of Cd were superscalar in the Ardisia gigantifolia Stapf of July 2013 Jinxiu county from Guangxi,the rest of batches were in line with standard requirements.The heavy metals of contents for Cu,Cd,Hg in Ardisia punctata Lindl were in line with standard requirements.The contents of As in Ardisia punctata Lindl were overweight including two batches,and the contents of Pb were overweight including four batches.(3)The TLC indentification methods of Ardisia gigantifolia Stapf and Ardisia punctata Lindl were reliable and repeatable,getted clear spot and had good separation effect.(4)Used HPLC determination the contents of Gallic acid,by method validation,they had good liner relationship and precision,repeatability and stablility,the recoveries were the range of 95%-105%.(5)The results of similarity were more than 0.9 in different batches of Ardisia gigantifolia Stapf and Ardisia punctata Lindl,each characteristic peak of resolution was better and in line with the requests of fingerprint.Conclusion: Integrated these experimental results,the quality standards were initially established in Ardisia gigantifolia Stapf and Ardisia punctata Lindl,to provide a scientific basic for Ardisia gigantifolia Stapf and Ardisia punctata Lindl of analysis and evaluation.The HPLC fingerprint research methods of Ardisia gigantifolia Stapf and Ardisia punctata Lindl were initially established,and these methods were hasaccuracy,reliability and strong features,the results of stability,precision and repeatability were in line with the requirements,could be used widely in Ardisia gigantifolia Stapf and Ardisia punctata Lindl of quality evaluation. |
