| chronic renal failure(CRF) another name is function failure Matasho chronic, It is a variety of causes of renal units seriously damaged and a Progressive decline in glomerular filtration rate, and the finalresult showed diffuse lesions with advanced kidney, such as acid-base inbalance, many of systemic involvement, and so on. With the increasingly severe environmental Pollution, unhealthy diety for examPle High salt and fat, Smoking and less moving. Disease incidence of nePhroPathy increasing. Because most Patients with chronic renal failure not treated early, renal damage accelerating and accessing to uremia quickly. The huge cost of treatment not only brought unbearable burden to families and society, but also reduce the quality of life. So, exPlore the treatment of chronic renal failure is very meaningful, to find simPle and effective method of treatment can imProve the quality of life of Patients and reduce the economic burden on families and society."Yi qi Pai du decoction" is come from the30years summarize by Professor Ou yangyonghong who is working in Qingyuan Chinese medicine HosPital. this decoction is has been widely used in the treatment of renal dysfunction clinical manifestations, laboratory Parameters, blood urea nitrogen, creatinine in Patients with elevated income Qingyuan City HosPital Agreement Prescription in imProving the mental state of the Patient, anemia, limb weakness and other symPtoms, lower serum creatinine, blood urea nitrogen, Proteinuria, urine occult blood and other asPects of the effect is significant. From1992Professor OuyangYonghong made using the "qing qi xie zhuo method" Treatment of chronic renal insufficiency, Wang Rui and other studies have found that "Yi qi Pai du decoction" could reduce microinflammation damage to the body and imProve malnutrition of Long term dialysis man. In2012"Yi qi Pai du decoction" treatment clinical studies with chronic renal insufficiency sPleen qi deficiency has become a subject of Pharmaceutical Administration of Guangdong Province, multi-Party data show that the imProvement of symPtoms and laboratory indicators. In view of this, hoPing to study in rats with chronic renal failure in exPerimental animals, and further confirm the role of Parties in imProving symPtoms of detoxification Qi rats and rats with reduced renal function, blood liPids, hemoglobin and other laboratory markers on and try to exPlore PrescriPtion mechanism, in order to better Promote this side, to Provide a new basis for the treatment of chronic renal failure.PurPose:To establish a rat model of chronic renal failure, observe the effects of chronic renal failure Fang Qi detoxification Great Heat the general situation, and the effect on serum creatinine, blood urea nitrogen, hemoglobin, liPids and renal Pathology.Methods:Select180-220gSPF grade40wistar rats, male and female, to give a solid diet and free access to water, feeding a week after the law, were randomly divided into4groups of10, to select a group of normal control group, and the remaining30to chronic renal insufficiency made modules, giving100mg/kg/d adenine gavage, after21days of continuous feeding successful model, select the successful model of rats weighing re-sorted by size, according to a computer-generated random numbers would have been rats in randomly assigned to three groups:model group, Niaoduqing group, Qi detox side group, based on the weight of normal adult rats and conversion, which Niaoduqing4. Og/kg according to the proportion of the group in accordance with the intragastric; Yiqi detox side groups by gavage23.38ml/kg to average each rat approximately4ml liquid, continuous administration for28days. Anesthesia, blood, serum creatinine, blood urea nitrogen, serum lipids, electrolytes and other indicators, observed renal pathology.Results:1, the modeling results:After modeling, model group rats compared with normal rats, poor mental state, unresponsive, color gray, reduced activity Compared with the normal weight group, the difference was statistically significant (P<0.05); however made between modules, weighing no significant difference (P>0.05). One model rats were randomly selected, heart blood, kidney function tests suggest significantly higher serum creatinine and blood urea nitrogen, renal pathological anatomy see kidney volume increases, the color of pale, showing " white kidneys ", HE staining of renal inter-ball, tubular, interstitial renal damage part of the organizational structure2, treatment outcome:(1) In general. Chronic renal gavage Qi Fang incomplete detoxification rat model generally superior to the control group of rats a good state of mind, quick response, food intake more than the shiny hair, but has not yet reached normal levels; and Niaoduqing group compared Qi Fang rats detox their mental state, responsiveness equivalent, but are more shiny hair, indicating Qi detoxification party group in terms of improving the general condition of rats with CRF slightly better than Niaoduqing group, but no difference.(2) improving body weight. Normal control group, the group and Yiqi detox Niaoduqing square body weight were gradually increased, a larger group of normal rise, rise slowly Niaoduqing group, the control group of rats in the experimental weight peaked the second week, then begin to decline further aggravate. After the experiment, Qi Fang detox group and the normal control group, model control group and Niaoduqing group were statistically significant difference compared (P<0.05); Niaoduqing group and model no significant difference in weight compared with the control group (P>0.05), there are differences (P<0.05) compared with Yiqi detox side group, indicating improvement in Niaoduqing CRF rats weighing less than Qi detox side effects aspects(3) the impact on renal function:Qi detox side compared with the model group, serum creatinine and blood urea nitrogen were reduced significantly (P<0.05), compared with the normal group, the two indexes were significantly higher (P<0.01), compared with Qi Fang detoxification Niaoduqing serum creatinine was significantly lower (P<0.05), blood urea nitrogen was no significant difference (P>0.05).(4) the impact on blood lipids:Qi detoxification party group compared with the control group in the TG and TC, no difference (P>0.05), but significantly higher with the control group (P<0.01). Niaoduqing group than in the control group was significantly lower (P<0.05), compared with Yiqi toxic side were significantly higher (P<0.05).(5) the impact on serum calcium, phosphorus:no significant difference (P>0.05) compared to serum calcium Fang Qi detoxification and normal control group, model control group were significantly different (P<0.01) compared with Niaoduqing significantly higher compared (P<0.05); regulate phosphorus, the Qi detox side with the normal control group, model control group and Niaoduqing group, the difference was not statistically significant (P>0.05).(6) the impact on hemoglobin:Qi detox side group with normal hemoglobin group had no significant difference (P>0.05), significantly higher compared with the model group and Niaoduqing group (P<0.01). In the red blood cell count, the Qi detox side group and normal control group, model control group and Niaoduqing group compared were large differences (P<0.01).(7) renal pathology observed:①appearance observed on kidney, Qi and kidney swelling detoxification party groups, the degree of thickening of the film is light compared with the model group, the control group compared with the model surface color depth, indicating that the party has a role in protecting renal function.②kidney weight/body weight ratio of impact. Adenine to rats with renal failure, swelling of the kidney, kidney weight/body weight ratio was significantly increased compared with normal group with a significant difference (P<0.01), Qi order to reduce kidney damage edema detoxification process, reducing kidney weight/body weight ratio (P<0.01), which was better than Niaoduqing group (P<0.05).③HE staining:rat kidney Qi detoxification group had a small amount of square tubular epithelial cell shedding, some renal tubular dilation, partially visible yellow crystalline performance of their kidney damage lighter than the control group.Conclusion:Qi detox side of adenine-induced chronic renal failure in rats with improved weight loss in rats, improve the general situation, improve kidney function, regulate blood lipids, correction of anemia, corrective action of calcium and phosphorus metabolism. In reducing serum creatinine, blood lipids and regulate calcium and phosphorus metabolism, better efficacy compared with Niaoduqing, with good prospects for development, but the specific mechanism of action remains to be further explored. |
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