Reduce Phlegm Xiaoliu Decoction Against Lung Cancer Multidrug Resistance Experimental Study

Objective： Discuss Reduce Phlegm Xiaoliu Decoction to reverse multi-drugresistance in mice with a tumor-burdened lewis lung cancer and themechanism of action, And for further in-depth study of drug resistance oflung cancer cells resistant to provide related basis.Methods：Will be made of1×107cells per ml of tumor and grout，Vaccination40C57BL/6mice (were male)， each with a syringe in the left axillaryhypodermic configured tumor size is about0.2ml. Randomly divided into4groups, namely, high dose group of Chinese materia medica high dose CTX+CTX group and blank control group. After half a month to be put to death,stripping transplanted tumor in mice, Weigh and calculate the YiLiuLv, andRT-PCR Elisa method were used respectively to detect transplanted tumorthe expression level of the P-gp GST in the organization, assessmentphlegm elimination tumor party in diffe rent test method of the effect ofreversing multi-drug resistance of tumor.Results：（1）Through the analysis of experiment groups of mice tumor weightand weight YiLiuLv can be obtained: In the expression of the weight, theexperimental mice with blank control group no significant difference, P>0.05); Traditional Chinese medicine (TCM) high dose CTX+YiLiuLv thanCTX group significantly higher blank control group, the difference wasstatistically significant, P <0.01（;2）RT-PCR Elisa detection of each groupof transplanted tumor cells multi-drug resistant index P-gp protein and theexpression of GSH was significant difference with the blank control group,P <0.01; Traditional Chinese medicine in high dose+CTX group P-gpexpression was lower than those of CTX group, the difference wasstatistically significant, P <0.01); GSH in CTX group and high dose groupof Chinese expression difference was not significant, P>0.05. Conclusion： Reduce Phlegm Xiaoliu Decoction can reduce tumor weight, toreduce the load, and can improve YiLiuLv, reverse multi-drug resistance oftumor, may be through by reduse the expression of P-gp protein and GSH,Promote tumor cell apoptosis and so on many kinds of ways to reversemulti-drug resistance of tumor.