The Study Of Gujin-xiaoliu Decoction To The Treatment Of Lung Cancer And Its Mechanism

Objectives:To study the clinical and experimental effect of the Gujin-Xiaoliu decoction to the middle and late period lung carcinoma.To discuss the mechanism of the Gujin-Xiaoliu decoction to lung carcinoma and provide a effective prescription for clinical therapy.Methods:The clinical part of this study absorbed 60 patients,who were allocated by random single-blinding assignment into two groups,one is therapeutic group(Gujin-Xiaoliu decoction group)and the other is control group(Ping-Xiao capsule group).The course of the treatment are two months and we observe the relevant symptoms,size of the tumor,the quality of life,the level of CEA and drug adverse reaction.The experimental part of this study was to observe the effect of medicated serum of Gujin-Xiaoliu decoction to SK-MES-1 cells through cell morphological effect,proliferation,apoptosis,expression of proliferation-assosiated protein Cyclin D1 expression of apoptosis-assosiated gene Bcl-2and Bax.Results:(1)The proportion of stability of tumor in therapeutic group was 80%,and the proportion in control group was 63.33%.The proportion had no statistical difference between the two groups(P>0.05),which showed therapeutic group didnot have superiority to control group at stabilizing the size of tumor.(2)The comparison through t-test between the two groups after treatment showed thar some symptoms(fatigued spirit and lack of strength,thirst,ect)had significant statistical difference(P<0.01),some symptoms(cough,short of breath,anorexia,etc)had statistical difference(P<0.05),and some symptoms(bloody sputum,thoracalgia,etc)had no statistical difference.The results showed Gujin-Xiaoliu decoction could improve some symptoms such as fatigued spirit and lack of strength,thirst,cough,short of breath,anorexia,but had no superiority to improve bloody sputum and thoracalgia.(3)The kps between the two groups had significant statistical difference(P<0.01)through t-test,this result showed that Gujin-Xiaoliu decoction had superiority to control group at improving the quality of life.(4)The comparision of weight between the two groups had significant statistical difference(P<0.01)through RxC listing X2-test,this result showed that Gujin-Xiaoliu decoction had significant superiority to control group at stabilizing and increasing weight.(5)The comparision of the level of CEA between the two groups had statistical difference(P<0.05)through t-test,this result showed that Gujin-Xiaoliu decoction had superiority to control group at decreasing the level of CEA.(6)We observed the effect of medicated serum of Gujin-Xiaoliu decoction to SK-MES-1 cells at 24h and 48h and found the dimension of some cells were smaller,pyknosis,cellular swelling,membra-nolysis and necrosis.We observed at 72h that the number of cells diminished significantly,and the density of cells diminished along with the higher dose of the medicated serum,a great deal of cells pyknosis,contract.become irregular,and swelling cells,rupture and necrosis cells also increased.(7)The herb serum containing Gujin-Xiaoliu decoction of low,middle and high dose showed growth inhibiting visibly to SK-MES-1 cells at 24h,48h and 72h,the growth inhibiting rate had significant statistical difference(P<0.01),so this result showed Gujin-Xiaoliu decoction had significant growth inhibiting effect to SK-MES-1 cells.And there were dose-dependent and time-dependent manner in some time point.The optimal effective time of herb serum containing Gujin-Xiaoliu decoction on cells was 72hours.The inhibition rate was37.95%.(8)The herb serum containing Gujin-Xiaoliu decoction showed apoptosis inducing ability to SK-MES-1 cells.SK-MES-1 cells were treated by Gujin-Xiaoliu decoction with low?median and high dose medicated serum,and compared with that of control group,there was a significant difference(P<0.01),Gujin-Xiaoliu decoction showed a significant apoptosis inducing effect to SK-MES-1 cells,and the effect showed a dose-dependent and time-dependent manner.The optimal effective time of herb serum containing Gujin-Xiaoliu decoction on cell was 72 hours.The apoptosis inducing rate was about 35.27%.(9)From the results of Western-blot,SK-MES-1 cells were treated by Gujin-Xiaoliu decoction with 48h,the expression of Cyclin D1 and Bcl-2 were reduced,the expression of Bax increased and the effect showed a dose-dependent manner.Conclusions:(1)Gujin-Xiaoliu decoction had superiority at stabilizing the size of tumor,improving the quality of life and stabilizing and increasing weight.(2)Gujin-Xiaoliu decoction could improve some symptoms such as fatigued spirit and lack of strength,thirst,cough,short of breath and anorexia.(3)Gujin-Xiaoliu decoction had significant growth inhibiting effect to SK-MES-1 cells.And there were dose-dependent and time-dependent manner in some time point.(4)Gujin-Xiaoliu decoction decreased the expression of Cyclin D1 of SK-MES-1 cells,which maybe one of the mechanism of Gujin-Xiaoliu's inhibiting effect.(5)Gujin-Xiaoliu decoction showed a significant apoptosis inducing effect to SK-MES-1 cells,and the effect showed a dose-dependent and time-dependent manner.(6)Gujin-Xiaoliu decoction increased the expression of Bax and decreased the expression of Bcl-2 of SK-MES-1 cells,which maybe one of the mechanism of Gujin-Xiaoliu's apoptosis inducing effect.