Anti Anxiety Effects Of Silktree Albizia Bark In Mice

Objective: Experiments in mice by behavior, observation of Albizzia julibrissinextract, effects of alcohol extract on the anxiety behavior in mice, and by enzymelinked immunosorbent assay of neurotransmitters in brain of mice to explore the barkof silktree water extract, alcohol extract of anti anxiety effect on mice through amechanism of Huan; skin water extract and alcohol extract extraction separationfurther, extract the different active site active site, the behavioral experiments toexplore the bark of silktree anti anxiety; through the study of the orthogonalexperimental design of Albizia julibrissin extract the best extraction process of antianxiety.Methods:â‘ The bark of silktree water extract anti anxiety experiment: male mice,administered different doses of Albizzia julibrissin extract, continuous intragastricadministration of5-HT in mice, and elevated plus maze, the camera obscuraobservation effect of water extract of Albizzia julibrissin on anxiety behavior in mice.Behavior to the whole mouse brain after the end of the experiment, determination ofmouse brain GABA, glutamate and serotonin neurotransmitter in5-HT by enzymelinked immunosorbent assay.â‘¡The bark of silktree alcohol extract anti anxiety experiment: male mice,administered different doses of alcohol extract of Albizzia julibrissin, continuousintragastric administration of5-HT in mice, and elevated plus maze, clear black boxobservation of Albizzia effects of alcohol extract on anxiety behavior in mice.â‘¢Study on anti anxiety the effective part of bark of silktree Albizzia: take thewater extract and alcohol extract with chloroform, ethyl acetate, respectively n-butanol gradient extraction, extracts were anti anxiety behavior experimental study.Behavioral ended by enzyme-linked immunosorbent assay in mouse brain GABA,glutamate and serotonin neurotransmitter in5-HT.â‘£Determination of Albizia julibrissin and high performance liquidchromatography (-)-syringaresinol-4-O-β-D furan Qin glycosyl-(1â†'2)-thecontent of β-D-glucopyranoside.⑤Through three factors and three levels orthogonal experiment design toexplore the bark of silktree water extraction optimum process.Conclusion:â‘ The bark of silktree water extract has obvious anti anxiety effects, itsanti anxiety effect mechanism and decrease Glu,5-HT content of brain in mice,increase the content of GABA in the brain of.â‘¡The bark of silktree alcohol extract anxiolytic effect.â‘¢The bark of silktree n-butanol extract has obvious anti anxiety effects, its antianxiety effect mechanism and decrease Glu,5-HT content of brain in mice, increaseâ‘£the content of GABA in the brain of. Wood ester compounds may be theeffective constituents of anti anxiety.⑤The bark of silktree water extract the optimum process for10times theamount of water, extracting for2times, each time1.5h.